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Anti-PKM2 (C-terminal) antibody produced in rabbit

品牌
Sigma-Aldrich
货号
SAB4200105
规格纯度
~1.5 mg/mL, affinity isolated antibody
参考价格
5705.99 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-PKM2 (C-terminal) antibody produced in rabbit
Anti-PK3
Anti-Pyruvate Kinase, MUSCLE (isoform M1)
Anti-CTHBP
Anti-TCB
Anti-THBP1 (thyroid hormone-binding protein, cytosolic)
Anti-PKM
Anti-OIP3 (OPA-interacting protein 3)
产品介绍:

产品说明

一般描述

Pyruvate Kinase (PK) is a key regulatory enzyme in glycolysis and has four known isoforms, namely, L, R, M1 and M2. PK isoform M1 is expressed during the development of the embryo and is the dominant isoform in cardiac, brain and skeletal muscle tissues. The M2 isoform has been linked to cancer metastasis

特异性

Anti-PKM2 (C-terminal) antibody is specific for human, rat, and mouse PKM2. In immunoblotting, detection of the PKM2 band is specifically inhibited by the PKM2 immunizing peptide.

应用

Anti-PKM2 (C-terminal) antibody produced in rabbit has been used in immunohistochemistry and immunocytochemistry.
Anti-PKM2 (C-terminal) antibody is suitable for use in immunoprecipitation (2-4 μg using A549 cell lysates) and western blot (approx. 60 kDa, 1-2 μg/mL using L8 and C2C12 cell lysates). The antibody may also be used for indirect immunofluorescence (2-4 μg using HeLa cells).

生化/生理作用

Phosphorylation of the M2 isoform at Tyr105 inhibits its activity and is common in human cancers, suggesting Tyr105 is a critical metabolic switch in cancer cells that promotes tumorigenesis. Tumor cells have been reported to exclusively overexpress the embryonic M2 isoform. The tumor metabolome is characterized by a high glycolytic turnover rate and tumor cells are able to proliferate under conditions of aerobic glycolysis, known as the Warburg effect. Knockdown of the M2 isoform in human cancer cell lines and its replacement by the M1 isoform has been shown to lead to reversal of the Warburg effect and reduced ability to form tumors in mouse xenografts.

外形

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRESNA.44

产品性质

生物来源rabbit
偶联物unconjugated
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
形式buffered aqueous glycerol solution
分子量 ~60 kDa
species reactivityrat, mouse, human
浓度~1.5 mg/mL
technique(s)immunoprecipitation (IP): 2-4 μg using A549 cell lysates
indirect immunofluorescence: 2-4 μg using HeLa cells
western blot: 1-2 μg/mL using L8 and C2C12 cell lysates
运输dry ice
储存温度−20℃
Gene Informationhuman ... PKM2(5315)
mouse ... Pkm2(18746)
rat ... Pkm(25630)

安全信息

储存分类代码12 - Non Combustible Liquids
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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