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Anti-Histone Deacetylase 7 (HDAC7) antibody, Mouse monoclonal

品牌
Sigma-Aldrich
货号
H6663
规格纯度
clone HDAC7-97, purified from hybridoma cell culture
参考价格
5045.46 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
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产品名称:
Anti-Histone Deacetylase 7 (HDAC7) antibody, Mouse monoclonal
Anti-HDAC7
产品介绍:

产品说明

一般描述

Histone deacetylases (HDACs) are competing enzymes, belonging to histone deacetylase family. There are two classes of HDACs with six to seven different types of HDACs proteins. HDAC1,HDAC2 and HDAC3 belong to Class I HDACs and HDAC4, HDAC6, and HDAC7 belong to Class II HDACs. Class I HDACs consists of a single deacetylase domain at the N-termini and diversified C-terminal regions, while Class II contains a deacetylase domain at C-terminal position. HDAC7 is predominantly found in the cell nucleus and slightly in cytoplasm. HDAC7 shuttles between these two regions and this is controlled by 14-3-3 protein and calmodulin-dependent kinase I (CaMKI)
Monoclonal Anti-Histone Deacetylase 7 (HDAC7) (mouse IgG1 isotype) is derived from the hybridoma HDAC7-97 produced by the fusion of mouse myeloma cells (NS1cells) and splenocytes from BALB/c mice. Histone deacetylases (HDACs) are part of transcriptional corepressor complexes. HDAC7 is a member of Mammalian HDAC family. It is localized mainly to the cell nucleus, it is also found in the cytoplasm.

应用

Monoclonal Anti-Histone Deacetylase 7 (HDAC7) antibody produced in mouse is suitable for:

  • immunocytochemistry
  • immunoprecipitation
  • indirect ELISA
  • microarray
  • western blot at a concentration of 0.25-0.5μg/mL using cell extracts of human embryonal kidney 293T cells expressing recombinant human HDAC7


Anti-Histone Deacetylase 7 (HDAC7) antibody, Mouse monoclonal has been used in:
  • chromatin immunoprecipitation
  • immunoblotting
  • immunocytochemistry
  • immunoprecipitation
  • ELISA

生化/生理作用

Shuttling of HDAC7 between the cell nucleus and the cytoplasm is controlled by a mechanism involving calmodulin dependent kinase I (CaMKI) and 14-3-3 proteins. The HDAC7 enzymatic activity depends on its interaction with the class I HDAC3, and the corepressors SMRT and N-CoR. HDAC7 also interacts with the transcriptional repressor BCL.
HDAC7 belongs to class II of HDAC family and combines with MEF2 group of transcription factors, which is facilitated by theN-terminal domain of HDAC7. This binding blocks the transcriptional activity and prevents myogenesis. They are involved in development and differentiation processes in various tissues such as skeletal, cardiac, and smooth muscle, the bone, the immune system, the vascular system, and the brain. These signal-dependent co-repressors phosporylate at the regulatory N-terminal domain resulting in nuclear export.

外形

Solution in 0.01 M phosphte buffered saline, pH 7.4, containing 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD06798700
NACRESNA.41

产品性质

质量水平200
生物来源mouse
偶联物unconjugated
抗体形式purified from hybridoma cell culture
antibody product typeprimary antibodies
克隆HDAC7-97, monoclonal
形式buffered aqueous solution
分子量antigen ~105 kDa
species reactivityhuman, mouse
technique(s)immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.25-0.5 μg/mL using recombinant protein from embryonal kidney 293T cells over-expressing human HDAC7
同位素/亚型IgG1
UniProt登记号Q8WUI4
运输dry ice
储存温度−20℃
Gene Informationhuman ... HDAC7(51564)
mouse ... Hdac7(56233)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKnwg
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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