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Anti-α1,2-Mannosidase IA antibody produced in rabbit

品牌
Sigma-Aldrich
货号
M3694
规格纯度
affinity isolated antibody, buffered aqueous solution
参考价格
4731.57 *本价格含增值税费
促销
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数量
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产品名称:
Anti-α1,2-Mannosidase IA antibody produced in rabbit
产品介绍:

产品说明

一般描述

α1,2-Mannosidase IA is a type II transmembrane Golgi-resident enzyme that belongs to class I α1,2-Mannosidases (glycosylhydrolase family 47). The highly conserved Class I α1,2-Mannosidases classified into three subgroups according to their enzymatic activities. α1,2-Mannosidase IA is predominantly detected in the juxtanuclear Golgi region. The three subgroups differ in their expression pattern, subcellular localization, and substrate specificity.

免疫原

synthetic peptide corresponding to amino acids 138-154 of human α1,2-mannosidase IA with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in mouse.

应用

Anti-α1,2-Mannosidase IA antibody produced in rabbit has been used in:

  • immunohistochemistry
  • immunoblotting
  • immunofluorescence

生化/生理作用

α1,2-Mannosidase IA belongs to the first subgroup out of the three subgroups of class I α1,2-Mannosidases. The first subgroup consists of yeast and human α 1,2-mannosidases of the endoplasmic reticulum that convert Man9GlcNAc2 to Man8GlcNAc2 isomer B. The second subgroup comprising of mammalian Golgi α1,2-Mannosidases IA, IB, and IC cleave Man9GlcNAc2 to Man5GlcNAc2 via Man8GlcNAc2 isomer A and C. The third subgroup of yeast and mammalian proteins do not show any activity on Man9GlcNAc2. Proteins from subgroup 1 and 3 have been implicated in ER quality control and in proteasomal degradation of misfolded glycoproteins. The Golgi mannosidases from the second subgroup may play a role in the ERAD (endoplasmic reticulum-associated degradation) of defective glycoproteins. The three subgroups have been found to pariticipate in the maturation of Asn-linked glycoproteins in the endoplasmic reticulum (ER) and Golgi complex.

外形

Solution in 0.01 M phosphate buffered saline containing 1% bovine serum albumin and 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD07784596
NACRESNA.41

产品性质

生物来源rabbit
质量水平200
偶联物unconjugated
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
形式buffered aqueous solution
分子量antigen ~73 kDa
species reactivityhuman, rat, mouse
technique(s)indirect immunofluorescence: 5-10 μg/mL using human HepG2 cells or rat NRK cells
western blot (chemiluminescent): 0.5-1 μg/mL using whole extract of mouse NIH3T3 cells
UniProt登记号P33908
运输dry ice
储存温度−20℃
Gene Informationhuman ... MAN1A1(4121)
mouse ... Man1a(17155)
rat ... Man1a1(294410)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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