产品说明
一般描述
Superoxide Dismutase (SOD) is a metalloprotein enzyme. It is widely distributed in the animal and plant kingdoms. The three common mammalian endogenous isozymes are the homodimeric copper-zinc SOD (Cu,ZnSOD, SOD1) found primarily in the cytosol, the homotetrameric glycosylated copper-zinc SOD, extracellular SOD (EC-SOD, SOD3), and the homotetrameric mitochondrial manganese SOD (MnSOD, SOD2). All three are nuclear-encoded. MnSOD is composed of four subunits, each containing one Mn2+.
免疫原
synthetic peptide corresponding to amino acid residues 183-199 of human superoxide dismutase (MnSOD) with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is conserved in many eukaryotes.
应用
The antibody may be detected by various immunochemical techniques including immunoblotting, co-immunoprecipitation assay (co-IP), immunoprecipitation, indirect immunofluorescence and immunohistochemistry.
生化/生理作用
Superoxide Dismutase (SOD) catalyzes the dismutation of the cytotoxic superoxide radical (O2 -) to molecular oxygen and hydrogen peroxide. Manganese SOD (MnSOD) has been proposed to function as a tumor suppressor by modulating the activity of redox-sensitive transcription factors and specific signal mediators. MnSOD is involved in detoxification.
外形
Solution in phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
法律信息
ExtrAvidin is a registered trademark of sigma-aldrich Co. LLC
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD06798545 |
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 偶联物 | unconjugated |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~24 kDa |
| species reactivity | human, rat |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10-20 μg/mL using biotin/ExtraAvidin®-peroxidase staining of human heart sections indirect immunofluorescence: 10-20 μg/mL using methanol-acetone-fixed rat NRK cells western blot: 1-2 μg/mL using human HeLa mitochondria extract and a chemiluminescent detection reagent western blot: 2-4 μg/mL using whole extract of rat brain and a chemiluminescent detection reagent |
| UniProt登记号 | P04179 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... SOD2(6648) rat ... Sod2(24787) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | nwg |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
