Anti-F-box/WD repeat-containing protein 7 Antibody

产品说明

一般描述

F-box/WD repeat-containing protein 7 (UniProt Q969H0; also known as Archipelago, F-box and WD-40 domain protein 7, F-box protein FBW7, F-box protein FBX30, F-box protein SEL-10, hAgo, hCdc4, Homolog of C elegans sel-10) is encoded by the FBXW7 (also known as AGO, CDC4, FBW7, FBX30, SEL10) gene (Gene ID 55294) in human. Polyubiquitination of proteins is mediated by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). There exist RING finger-type, HECT-type, and U-box type of E3 ligases. The F-box/WD repeat-containing protein 7 functions as an adaptor component of the Skp1, Cullin, F-box (SCF) and mediates substrates recruitment to the Cullin-RING E3 ubiquitin ligase (CRL) complex. Cyclin E, c-Myc, c-Jun, Notch1, SREBP, mTOR, c-Myb, MCL1, NFκB2, and GATA3 are all known substrates targeted by Fbxw7 for ubiquitin-proteasome system- (UPS-) mediated degradation.

特异性

Although immunogen sequence is present in all three spliced isoforms, both target band size by Western blotting and cytoplasmic staining pattern by immunohistochemistry are consistent with the detection of isoform 2 (Archipelago beta, FBW7beta, 69K) by this polyclonal antibody.

免疫原

KLH-conjugated linear peptide corresponding to human F-box/WD repeat-containing protein 7.
Epitope: Internal

应用

Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected F-box/WD repeat-containing protein 7 in human cerebellum and human pancreas tissue.
This Anti-F-box/WD repeat-containing protein 7 Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of F-box/WD repeat-containing protein 7.
Research Sub Category
Nuclear Receptors
Research Category
Epigenetics & Nuclear Function

质量

Evaluated by Western Blotting in MCF-10A human breast epithelial cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected F-box/WD repeat-containing protein 7 in 10 µg of human breast cancer MDA-MB-231 and non-tumorigenic MCF-10A breast epithelial cell lysates.

目标描述

~70 kDa observed. 79.7 kDa (isoform 1; Archipelago alpha, FBW7alpha, 110K, common), 70.3 kDa (isoform 2; Archipelago beta, FBW7beta, 69K), and 66.1 kDa (isoform 3; Archipelago gamma, FBW7gamma, Hippocampal) calculated.

外形

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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