您好,欢迎来到试剂仪器网! [登录] [免费注册]
试剂仪器网
位置:首页 > 品牌 > Sigma-Aldrich > Anti-Vimentin Antibody, clone VIM 3B4

Anti-Vimentin Antibody, clone VIM 3B4

品牌
Sigma-Aldrich
货号
CBL202
规格纯度
clone VIM 3B4, Chemicon®, from mouse
参考价格
5807.07 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-Vimentin Antibody, clone VIM 3B4
产品介绍:

产品说明

特异性

Highly specific for the intermediate filament protein vimentin which is present in all cells of mesenchymal origin. This antibody is positive with cells of mesenchymal derivation, including endothelial cells and certain smooth muscle cells of the vascular tract, fibroblasts, connective tissue, all types of blood cells, including thymocytes, interstitial and testicular Sertoli cells, ovarian follicle cells. It can also be used to detect coexpression of vimentin in combination with other intermediate filament proteins. VIM 3B4 has turned out to be the most avid monoclonal antibody to vimentin. Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma. The binding region of monoclonal antibody VIM3B4 has been characterized by Bohn et al. (1992). According to these authors, the epitope has been localized on the alpha-helical part of vimentin (rod domain coil 2). Due to an aa substitution at position of aa 353 in murine vimentin (that could explain for the weak cross-reaction of the antibody with murine vimentin) they were able to narrow down the binding region around position 353. These findings were confirmed by truncation mutagenesis experiments using human vimentin (Rogers et al., 1995). Polypeptide reacting: MW 57 kDa intermediate filament protein (vimentin) of mesenchymal cells.

免疫原

Vimentin purified from bovine lens

应用

Research Category
Cell Structure
Western blot
Immunohistochemistry: 1:100; Frozen and paraffin-embedded tissue sections with a minimum of a one hour incubation (longer for paraffin); protease pretreatment is recommended for paraffin-embedded sections.
Immunofluorescence
ELISA

Optimal working dilutions must be determined by the end user.
This Anti-Vimentin Antibody, clone VIM 3B4 is validated for use in ELISA, IF, IH(P), WB for the detection of Vimentin.
Research Sub Category
Cytoskeleton

目标描述

57 kDa

外形

Protein A Purified immunoglobulin presented as lyophilized material. Reconstitute with 1ml distilled water. Final solution is PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide.
Format: Purified
Protein A purified

储存及稳定性

Maintain at 2–8°C for up to 12 months from date of receipt.

分析说明

Control
Positive in RD cells, glioma cells, fibroblasts (SV-80), and MDCK cell line

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆VIM 3B4, monoclonal
species reactivitycanine, amphibian, bovine, monkey, chicken, human
manufacturer/tradenameChemicon®
technique(s)ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
同位素/亚型IgG2a
NCBI登记号NM_003380.2
UniProt登记号P08670
运输wet ice
Gene Informationhuman ... VIM(7431)

安全信息

象形图GHS07
警示用语:Warning
危险声明H312 + H332 - H412
预防措施声明P261 - P271 - P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312
危险分类Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3
储存分类代码11 - Combustible Solids
WGKWGK 3

Sigma-Aldrich

推荐产品