产品说明

一般描述

High affinity immunoglobulin epsilon receptor subunit gamma (UniProt P20411; also known as Fc-epsilon RI-gamma, Fc receptor gamma-chain, FceRI gamma, FcR gamma, IgE Fc receptor subunit gamma) is encoded by the Fcer1g (also known as Fce1g) gene (Gene ID: 25441) in rat species. FceR1 gamma is a single-pass type I membrane protein that associates with a variety of FcR alpha chains to form a functional signaling complex that regulates several aspects of the immune response. The gamma subunit has a critical role in allowing the IgE Fc receptor to reach the cell surface. FceRI gamma is found exclusively on mast cells and basophils. In rodents Fc epsilon RI is a tetrameric complex of non-covalently attached subunits: one IgE-binding alpha subunit, one beta subunit and a dimer of disulfide-linked gamma subunits. When multivalent allergens bind to the receptor-bound IgE, the consequent aggregation of the receptors leads to the release of mediators responsible for allergic symptoms. (Ref.: Blank, U., et al. (1989). Nature 337(6203); 187-189).

特异性

Other species cross-reactivity not tested.
Recognizes and is specific for FcεRI, γ-subunit, MW 10kDa.

免疫原

KLH-conjugated linear peptide corresponding to 7 amino acids from the C-terminal region of rat FceRI gamma subunit.

应用

Anti-FceRI, gamma subunit, Cat. No. 06-727, is a rabbit polyclonal antibody that detects FceRI, subunit and has been tested for use in Flow Cytometry and Western Blotting.
Flow Cytometry Analysis: 0.1 µg from a representative lot detected FceRI, gamma subunit in rat basophilic leukemia cells.

Research Sub Category
Immunoglobulins & Immunology
Research Category
Inflammation & Immunology

质量

Evaluated by western blot on RIPA lysate of RBL cells.

Western Blot Analysis: 0.5-2 μg/mL of this antibody detected FcεRI, γ-subunit in RIPA lysate of RBL cells.

目标描述

30 kDa

外形

Purified rabbit polylclonal IgG in buffer containing 70% storage buffer (0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide) and 30% glycerol.
Format: Purified
Protein A purified

储存及稳定性

Stable for 1 year at from date of receipt.

分析说明

Control
RBL cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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