Anti-TrkA Antibody

产品说明

一般描述

TrkA, apparent molecular weight 140 kDa, is a high affinity nerve growth factor (NGF). The Trk proto-oncogene family contains four members, TrkA, TrkB, TrkC, and TrkE, which are variably expressed throughout the central and peripheral nervous systems. TrkA binds to nerve growth factor (NGF) and autophosphorylates on tyrosine residues (Tyr490, Tyr674, Tyr675, Tyr751 and Tyr785) to activate multiple downstream effector proteins. Phosphorylation at Tyr490 is required for Shc association and subsequent activation of the Ras-MAP kinase-signaling cascade, which leads to activation of Elk-1-dependent gene transcription and neurite growth. Phosphorylations at Tyr674 and Tyr675 lie within the catalytic domain of TrkA tyrosine kinase and reflect Trk kinase activity. Additionally, phosphorylation at Tyr751 is required for PI3-kinase association and activation of the Akt signaling cascade.

特异性

Reactivity with other species has not been confirmed.
Recognizes TrkA, Mr 140 kDa. Does not cross-react with TrkB or TrkC.

免疫原

Rabbits immunized with bacterially expressed purified protein corresponding to the entire extracellular domain of rat TrkA receptor.

应用

Research Sub Category
Neurotransmitters & Receptors

Neurodegenerative Diseases
Research Category
Neuroscience
Anti-TrkA Antibody detects level of TrkA & has been published & validated for use in IC, IP & WB.
Immunoprecipitation:
4 μg of a previous lot immunoprecipitated TrkA from 500 μg of PC-12 RIPA lysate.

Immunocytochemistry:
10 μg/mL of a previous lot of this antibody showed positive immunostaining for TrkA in PC-12 cells fixed with 4% paraformaldehyde.

质量

Routinely evaluated by Western Blot on RIPA lysates from human A431 cells, mouse 3T3 or rat PC-12 cell lysates.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected TrkA in RIPA lysates from human A431 cells; a previous lot detected TrkA in mouse 3T3 and rat PC-12 cell lysates.

目标描述

140 kDa

联系

Replaces: CBL770

外形

Format: Purified
Protein A purified
Purified rabbit IgG in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, 0.1% sodium azide. Frozen at -20°C.

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.

分析说明

Control
EGF-stimulated A431 cel lysate, rat brain tissue. Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

产品性质

安全信息