产品说明
一般描述
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.
MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.
应用
Recommended for use in a carbon dioxide-free culture system.
其他说明
This product lacks L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser and L-Gln.
May also be supplemented with HEPES to raise the buffering range.
重悬
添加 0.292g/L
基本信息
NACRES | NA.75 |
产品性质
质量水平 | 400 |
无菌性 | sterile-filtered |
形式 | liquid |
technique(s) | cell culture | mammalian: suitable |
杂质 | endotoxin, tested |
web metadata keyword.default | classic media, classical media, MEM, classic media, classical media, FBS |
组分 | glucose: 1.0 g/L phenol red: 0.011 g/L L-glutamine: no NaHCO3: 0.85 g/L |
运输 | ambient |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich