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Minimum Essential Medium Eagle

低限量Eagle培养基

品牌
Sigma-Aldrich
货号
M2414
规格纯度
Modified, with Earle′s salts and reduced NaHCO3. without L-glutamine, liquid, sterile-filtered, suitable for cell culture
参考价格
663.31 *本价格含增值税费
促销
服务
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数量
-+
产品名称:
Minimum Essential Medium Eagle
MEM
低限量Eagle培养基
产品介绍:

产品说明

一般描述

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.

MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.

应用

Recommended for use in a carbon dioxide-free culture system.

其他说明

This product lacks L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser and L-Gln.
May also be supplemented with HEPES to raise the buffering range.

重悬

添加 0.292g/L L-谷氨酰胺。

基本信息

NACRESNA.75

产品性质

质量水平400
无菌性sterile-filtered
形式liquid
technique(s)cell culture | mammalian: suitable
杂质 endotoxin, tested
web metadata keyword.defaultclassic media, classical media, MEM, classic media, classical media, FBS
组分glucose: 1.0 g/L
phenol red: 0.011 g/L
L-glutamine: no
NaHCO3: 0.85 g/L
运输ambient
储存温度2-8℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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