产品说明
一般描述
Bromothymol Blue is a sulfonated hydroxyquinone in acidic solutions. In alkaline solutions, it is present as a dianion that is blue with a larger conjugated system than the yellow hydroxyquinone. Both forms are lipophilic. It is also known as bromthymol blue or dibromothymolsulfonphthalein.
应用
Bromothymol blue has various applications in biology and medicine. It is suitable for demonstrating fungal hyphae within plant roots. It is used as a vital stain to trace the movement of fluids from the lymph. It is added to an agar gel microbial culture medium for enumeration of Bacillus cereus. Bromothymol blue is also used as a pH indicator due to its precise color transition properties at neutrality.
包装
100 mL in glass bottle
生化/生理作用
Bromothymol Blue is a pH indicator which changes color from yellow (pH 6.0) to blue (7.6). It belongs to the class of sulfonephthalein dyes.
基本信息
经验(实验)分子式 | C27H27Br2NaO5S |
分子量 | 646.36 |
MDL编号 | MFCD00077263 |
PubChem化学物质编号 | 24859144 |
NACRES | NA.47 |
产品性质
质量水平 | 200 |
形式 | liquid |
浓度 | 0.04 wt. % in H2O |
pH范围 | 6.0 - 7.6, yellow to blue |
密度 | 0.99 g/mL at 25 ℃ (lit.) |
λmax | 615 nm |
ε (extinction coefficient) | ≥13000 at 304-310 nm in 0.1 M NaOH at 0.005 g/L ≥8000 at 391-397 nm in 0.1 M NaOH at 0.005 g/L |
application(s) | diagnostic assay manufacturing hematology histology |
储存温度 | room temp |
SMILES string | [Na+].CC(C)c1cc(c(C)c(Br)c1O)\C(=C2/C=C(C(C)C)C(=O)C(Br)=C2C)c3ccccc3S([O-])(=O)=O |
InChI | 1S/C27H28Br2O5S.Na/c1-13(2)18-11-20(15(5)24(28)26(18)30)23(17-9-7-8-10-22(17)35(32,33)34)21-12-19(14(3)4)27(31)25(29)16(21)6;/h7-14,30H,1-6H3,(H,32,33,34);/q;+1/p-1/b23-21+; |
InChI key | NMKFVGALBGZKGW-FKWCIMQXSA-M |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
Sigma-Aldrich