产品说明
特异性
Recognizes phospho-EGFR (Tyr845).
Predicted to cross-react with mouse and rat based on sequence homology (100%), but have not been tested.
免疫原
Peptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-EGFR (Tyr845)
应用
Research Sub Category
Growth Factors & Receptors
Research Category
Signaling
This Anti-phospho-EGFR (Tyr845) Antibody is validated for use in IC, IF, IH, WB for the detection of phospho-EGFR (Tyr845).
Immunoblot Analysis: A 1:1000 dilution of this lot detected phospho-EGFR (Tyr845) in RIPA lysates from EGF stimulated A431 cells.
质量
Routinely evaluated by immunoblot.
目标描述
185 kDa
外形
ImmunoAffinity Purified
Affinity Purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.
储存及稳定性
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
分析说明
Control
EGF stimulated A431 cells
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | affinity purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | polyclonal |
纯化方式 | affinity chromatography |
species reactivity | human |
manufacturer/tradename | Upstate® |
technique(s) | immunocytochemistry: suitable immunofluorescence: suitable immunohistochemistry: suitable western blot: suitable |
NCBI登记号 | NM_005228.3 NM_201282.1 NM_201283.1 NM_201284.1 |
UniProt登记号 | P00533 |
运输 | dry ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich