Anti-eIF4G Antibody

产品说明

一般描述

eIF4F is a translation initiation factor containing three subunits: eIF4A, eIF4E, and eIF4G. This complex is a key component in promoting ribosomal binding to the mRNA. eIF4G interacts with both eIF4A and eIF4E though its carboxy and amino terminal domains, respectively. eIF4E binds to the cap structure of the mRNA, while the ATP-dependent RNA helicase eIF4A unwinds the secondary structure of the 5’ UTR. eIF4G plays a critical role in coordinating the assembly of the eukaryotic initiation factors. It acts as a bridge to bind the cap-bound eIF4E, eIF4AI&II, the eIF3 complex, PABP1, and the kinase Mnk1 (Lejeune, F., et al., 2004) in a large eukaryotic initiation factor complex

特异性

Not tested on other species.
This antibody detects eIF4G at its N-terminal region.

免疫原

Synthetic peptide corresponding to N-terminal region of human eIF4G.
Epitope: N-terminus

应用

Research Sub Category
RNA Binding Protein (RBP)

Insulin/Energy Signaling
Anti-eIF4G Antibody is an antibody against eIF4G for use in WB & IF.
Research Category
Signaling

质量

Evaluated by western blot on HeLa cytoplasmic extract.

Western Blot Analysis: 1:1,000 dilution of this antibody was used to detect eIF4G in HeLa cytoplasmic extract and lysates from 293T and S10 cells.

目标描述

220 kDa

外形

Rabbit polyclonal serum with 0.05% NaN3.

储存及稳定性

Stable for 1 year at -20ºC from date of receipt.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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