Anti-phospho-Upf1 (Ser1127) Antibody

产品说明

一般描述

Regulator of nonsense transcripts 1 (UniProt: Q92900; also known as ATP-dependent helicase RENT1, Nonsense mRNA reducing factor 1, NORF1, Up-frameshift suppressor 1 homolog, hUpf1) is encoded by the UPF1 (also known as KIAA0221, RENT1) gene (Gene ID: 5976) in human. Upf1 is a member of the DNA2/NAM7 helicase family. It is a RNA-dependent helicase and ATPase required for nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. It is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation. It is phosphorylated by SMG1 and phosphorylation is considered as an essential step in NMD and is required for formation of mRNA surveillance complexes. Phosphorylated Upf1 is recognized by EST1B/SMG5, SMG6, and SMG7, which provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways. A hyper-phosphorylated form is targeted to the P-body, while unphosphorylated protein is distributed throughout the cytoplasm. The ATPase activity of Upf1 is required for disassembly of mRNPs undergoing NMD and is also essential for embryonic viability. Two isoforms of Upf1 have been reported that are produced by alternative splicing. Upf1 contains a UPF1-type zinc-finger domain (aa 121-272) and a nucleotide-binding domain (aa 506-510).

特异性

This rabbit polyclonal antibody detects Regulator of nonsense transcripts 1 (RENT1) in multiple species. It targets an epitope within 9 amino acids surrounding phosphorylated Ser1127.

免疫原

KLH-conjugated linear peptide corresponding to Upf1 phosphorylated at Ser1127.
Epitope: Phosphorylated Ser1127

应用

Anti-phospho-Upf1 (Ser1127), Cat. No. 07-1016, is a highly specific rabbit polyclonal antibody that targets Regulator of nonsense transcripts 1 (RENT1) phosphorylated at Ser1127 and has been tested in Immunoprecipitation, peptide Inhibition Assay, and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected phospho-Upf1 (Ser1127) in 10 µg of A431 treated with Calyculin A and Okadaic Acid.

Peptide Inhibition Analysis: A 1:1,000 dilution from a representative lot blocked phospho-Upf1 (Ser1127) in NIH3T3 treated with Calyculin A and Okadaic Acid.

Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated phospho-Upf1 (Ser1127) in NIH3T3 treated with Calyculin A and Okadaic Acid.

质量

Evaluated by Western Blotting in NIH3T3 treated with Calyculin A and Okadaic Acid.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-Upf1 (Ser1127) in 10 µg lysate from NIH3T3 treated with Calyculin A (50 nM) and Okadaic Acid (500 nM) for 30 minutes.

目标描述

~140 kDa observed. 125 kDa calculated.

外形

Affinity purified

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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