产品说明
一般描述
PP2A is a heterotrimeric type 2 Ser/Thr phosphatase, composed of a structural subunit (A), a regulatory subunit (B), and a catalytic subunit (C). The structural A and catalytic C subunits form the constitutive core of the enzyme, which associates with one of a large number of regulatory B subunits. There are multiple isotypes (encoded by distinct genes) of the B subunit, grouped into three families: B/PR55, B′/PR61, and B′′/PR72 . The prototypical B (or PR55) family of subunits is encoded by three genes, Balpha, Bbeta and Bgamma . The B′ (or B56) family consists of at least seven splice variants derived from five genes. RNA′s for the two members of the B′′ family, PR72 and PR130, arise by alternative splicing or use of alternate transcription start sites from the same gene. Primary structures as well as functions of B and B′ subunits are conserved from yeast to mammals. PTPA is a member of the B′ family that is required for activation of PP2A by an undefined mechanism. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and its high sensitivity to Okadaic Acid. It is also thought to be the principal downmodulator of Ser/Thr kinase cascades.
特异性
Recognizes Protein Phosphatase 2A/B. Minimal cross reactivity against 2A/B.
免疫原
The immunogen used to the purified peptide conjugated to KLH corresponding to the sequence NH2-Cys-Gly-Glu-Glu-Asp-Ile-Asp-THr-Arg- Lys-Ile-Asn-Asn-Ser-Phe-COOH.
应用
Anti-PP2A Antibody, B β subunit detects level of PP2A & has been published & validated for use in WB, IH(P).
Research Sub Category
PI3K, Akt, & mTOR Signaling
Kinases & Phosphatases
Research Category
Signaling
质量
Routinely evaluated by Western Blot on NIH/3T3 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected PP2A/B BETA on 10 μg of NIH/3T3 lysates.
目标描述
~55 kDa
联系
Replaces: AB4086
外形
Affinity purified
Purified rabbit polyclonal IgG provided as 0.2 µm sterile filtered solution in phosphate buffered saline with 0.08% sodium azide.
储存及稳定性
Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
分析说明
Control
NIH/3T3 Cell Lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | affinity purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | polyclonal |
纯化方式 | affinity chromatography |
species reactivity | human, mouse, bovine, rat |
technique(s) | immunohistochemistry: suitable (paraffin) western blot: suitable |
同位素/亚型 | IgG |
NCBI登记号 | NM_001009552 |
UniProt登记号 | P62714 |
运输 | wet ice |
Gene Information | human ... PPP2CB(5516) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich