产品说明
一般描述
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Di- and tri-methylation of histone H3 are gene silencing marks, while removal of these marks by the demethylase genes Jmjd1a and Jmjd2c leads to gene activation. This product is a conjugated version of Cat. No. 07-449.
免疫原
Linear peptide corresponding to Histone H3 trimethylated at Lys27.
应用
This Anti-trimethyl Histone H3 (Lys27) antibody is validated for use in ICC for the detection of trimethyl Histone H3 (Lys27).
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
质量
Evaluated by Immunocytochemistry Analysis: in HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected trimethyl Histone H3 (Lys27) in HeLa cells.
Alexa Fluor® is a registered trademark of Life Technologies
目标描述
~17 kDa observed (Refer to Cat. No. 07-449)
外形
Protein A purified
Purified rabbit conjugate antibody in buffer containing PBS and 15 mg/ml BSA with 0.1% Sodium Azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
法律信息
ALEXA FLUOR is a registered trademark of Life Technologies
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 偶联物 | ALEXA FLUOR® 647 |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| species reactivity | human |
| species reactivity (predicted by homology) | mouse (based on 100% sequence homology) |
| 浓度 | 0.5 mg/mL |
| technique(s) | immunocytochemistry: suitable |
| NCBI登记号 | NM_003493 |
| UniProt登记号 | Q16695 |
| 运输 | wet ice |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
