产品说明
一般描述
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
特异性
Histone H3 dimethylated on lysine 9.
免疫原
Epitope: H3K9me2
KLH-conjugated, 2X-branched synthetic peptide containing the sequence ..RKme2STG.., in which Kme2 corresponds to dimethyl-lysine at residue 9 of human Histone H3.
应用
Research Sub Category
Nuclear Receptors
Anti-dimethyl-Histone H3 (Lys9) Antibody, AlexaFluor
Research Category
Epigenetics & Nuclear Function
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected dimethyl-Histone H3 (Lys9) in HepG2 cells.
The unconjugated antibody (Cat. No. 07-441) is shown to be suitable also for Western blotting, dot blot, immunocytochemistry, and peptide inhibition assay (peptide blocking) applications.
质量
Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected dimethyl-Histone H3 (Lys9) in HeLa cells.
目标描述
~17 kDa observed
外形
Purified rabbit antibody conjugate in PBS with 15 mg/ml BSA and 0.1 % sodium azide.
Protein A purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to lot specific datasheet.
法律信息
ALEXA FLUOR is a registered trademark of Life Technologies
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
偶联物 | ALEXA FLUOR® 488 |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | polyclonal |
species reactivity | human, mouse |
species reactivity (predicted by homology) | yeast (based on 100% sequence homology), rat (based on 100% sequence homology), chicken (based on 100% sequence homology) |
technique(s) | immunocytochemistry: suitable |
NCBI登记号 | NM_003493 |
UniProt登记号 | Q16695 |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich