TSKgel^® Ether-5PW Guardgel Kit

产品说明

一般描述

TSKgel Guardgel kits contain:

• 5 mL Guardgel packing material
• Column holder, consisting of external reducing union, cap, frit, 2 ferrules
• Column, 25 mm x 9.5 mm OD x 6 mm ID, 2 μm frit at 10 mm depth, 1/16"" exit
• 2 Replacement frits
• Capillary tubing, 5 cm x 1/16"" OD x 0.01"" ID
• Nuts and ferrules, 10-32 threads for 1/16"" tubing, 2 each
• All hardware is 316 stainless steel

Dimensions for Guardgel kits for 21.5 mm ID columns:

• Column, 35 mm x 12.75 mm OD x 10 mm ID, 10 μm frit at 20mm depth, 1/16"
• Capillary tubing, 5 cm x 1/16"" OD x 0.02"" ID

Both hydrophobic interaction chromatography (HIC) and reversed-phase liquid chromatography separate on the basis of protein hydrophobicity and allow selective binding and desorption of proteins. However, HIC operates at significantly lower binding energy and uses aqueous mobile phases. These characteristics provide a gentle technique that is less likely to disturb protein conformation. As a result, HIC generally provides better activity recovery.
Three resin-based TSKgel HIC columns provide a range of hydrophobicities for chromatographic optimization. TSKgel Phenyl-5PW columns are most hydrophobic, followed by Ether-5PW, while the surface of Butyl-NPR columns is most polar or least hydrophobic. Ammonium sulfate concentration can be minimized by using a more hydrophobic packing such as Phenyl-5PW. We recommend the more polar Ether-5PW column for purifying more hydrophobic proteins. Ether-5PW and Phenyl-5PW packings are based on TSKgel G5000PW resin - 10 micron particles with 1000  pores. Butyl-NPR packing is prepared from 2.5 micron nonporous particles, allowing for rapid analyses and semi-micropreparative applications. All three column types can be cleaned with 0.2 M NaOH. Scale-up can be performed by using corresponding Toyopearl bulk resins.

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法律信息

TSKgel is a registered trademark of Tosoh Corporation

产品性质

物料	PEEK
产品线	TSKgel®
manufacturer/tradename	Tosoh Ether-5PW, 08643
参数	≤4 M salt concentration ≤50% organic solvent 10-50 ℃ temperature
technique(s)	LPLC: suitable
长度 × 内径	2.5 cm × 6 mm
基质	polymer particle platform
基质活性基团	ether phase
粒径	20 μm
操作pH	2 - 12
分离技术	hydrophobic interaction (HIC)