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位置:首页 > 品牌 > Sigma-Aldrich > Ep-CAM/Epithelial Specific Antigen (Ber-EP4) Mouse Monoclonal Antibody

Ep-CAM/Epithelial Specific Antigen (Ber-EP4) Mouse Monoclonal Antibody

品牌
Sigma-Aldrich
货号
248M-9
包装型号
规格纯度
参考价格
1864.22 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Ep-CAM/Epithelial Specific Antigen (Ber-EP4) Mouse Monoclonal Antibody
产品介绍:

产品说明

一般描述

Ep-CAM/Epithelial Specific Antigen consists of two glycoproteins, 34 and 39 kDa, sometimes designated epithelial antigen, epithelial specific antigen, and epithelial glycoprotein. In paraffin sections, the protein is detected with mAbs like Ber-EP4 and MOC-31. The glycoproteins are located on the cell membrane surface and in the cytoplasm of virtually all epithelial cells with the exception of most squamous epithelia, hepatocytes, renal proximal tubular cells, gastric parietal cells and myoepithelial cells. However, focal positivity may be seen in the basal layer of squamous cell epithelium of endoderm (e.g., palatine tonsils) and mesoderm (e.g., uterine cervix). In liver lesions like hepatitis and cirrhosis, the hepatocytes frequently become Ep-CAM positive. Normal mesothelial cells are Ep-CAM negative, but may express focal reaction when undergoing reactive changes. Mesenchymal cells and cells from the neural crest are negative, with the exception of olfactory neurons. Ep-CAM is found in the large majority of adenocarcinomas of most sites (50-100% in various studies; as well as neuroendocrine tumours, including small cell carcinoma. Renal cell carcinoma and hepatocellular carcinoma stain in about 30% of the cases. Squamous cell carcinoma of endoderm and mesoderm is usually Ber-EP4 positive, while that of ectoderm is negative. Basal cell and basosquamous carcinoma are Ber-EP4 positive in almost all cases. Choroid plexus papilloma and carcinoma are usually negative. Malignant mesothelioma (epithelioid and biphasic) is Ep-CAM positive in 4-26% of the cases. The staining is usually focal, but may occasionally be widespread. Synovial sarcoma (epitheloid and biphasic) and desmoplastic small round cell tumour stain for Ep-CAM in most cases. Seminoma, embryonal carcinoma, yolk sac tumour and choriocarcinoma reveal Ber-EP4 positivity in a minor proportion of cases. Among neural tumours, Ep-CAM positivity is seen only in olfactory neuroblastoma. Ep-CAM can be of great help in the differential diagnosis of malignant involvement in the peritoneal and pleural cavities. The lack of reactivity in the majority of malignant mesothelioma can in an appropriate panel be utilized in the discrimination between this tumour and adenocarcinoma. As for a series of anti-epithelial antibodies, Ber-EP4 or MOC-31 may may be used in the demonstration of epithelial cell differentiation in cases where anti-cytokeratins do not produce a clear-cut positivity or in cases where a false positivity for cytokeratin cannot be excluded, such as in submesothelial cells.

质量


IVD

IVD

IVD

RUO

联系

Ep-CAM Positive Control Slides, Product No. 248S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

外形

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

制备说明

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其他说明

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

法律信息

Cell Marque is a trademark of sigma-aldrich Co. LLC

基本信息

NACRESNA.41

产品性质

质量水平500
100
生物来源mouse
偶联物unconjugated
抗体形式culture supernatant
antibody product typeprimary antibodies
克隆Ber-EP4, monoclonal
描述For In Vitro Diagnostic Use in Select Regions (See Chart)
形式buffered aqueous solution
species reactivityhuman
包装vial of 0.1 mL concentrate (248M-94)
vial of 0.5 mL concentrate (248M-95)
bottle of 1.0 mL predilute (248M-97)
vial of 1.0 mL concentrate (248M-96)
bottle of 7.0 mL predilute (248M-98)
manufacturer/tradenameCell Marque
technique(s)immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:200
同位素/亚型IgG1κ
控制colon carcinoma
运输wet ice
储存温度2-8℃
可视化cytoplasmic

Sigma-Aldrich

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