产品说明
一般描述
Lin28 is a developmentally regulated RNA binding protein (RBP). Its down regulation is mediated in part by Lin4 and miR-125b, the mammalian micro-RNA homolog of Lin4. It is known to be negatively regulated by retinoic acid and act as a translational enhancer by driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis by increasing the number of initiation events per mRNA molecule. Lin28 is necessary and sufficient for selectively blocking the cleavage processing of pri-let-7 miRNAs in embryonic cells. Lin28 is a negative regulator of miRNA biogenesis and may play a role in blocking miRNA-mediated differentiation in stem cells and in certain cancer. The mechanism by which miR-125b represses Lin28 in mammalian cells appears to involve reductions not only in translational efficiency, but also in mRNA abundance. Lin28 has shown to be essential for skeletal muscle differentiation through the translational upregulation of IGF2 expression. It is known to be expressed in testis, placenta, and embryonic stem (ES) cells.
特异性
Other homologies: Rat and Mouse 98% and 97% sequence homology
免疫原
Histidine-tagged recombinant protein corresponding to human Lin28.
应用
Anti-Lin28 Antibody is a Rabbit Polyclonal Antibody for detection of Lin28 also known as Protein lin-28 homolog A & has been validated in WB.
质量
Evaluated by Western Blot in NT2/D1 cell lyate.
Western Blot Analysis: 1:5,000 dilution of this antibody detected Lin28 in 10 µg of NT2/D1 cell lysate.
目标描述
~28 kDa observed
外形
Affinity purified
分析说明
Control
NT2/D1 cell lyate
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | affinity isolated antibody |
antibody product type | primary antibodies |
克隆 | polyclonal |
纯化方式 | affinity chromatography |
species reactivity | human |
technique(s) | western blot: suitable |
NCBI登记号 | NP_078950 |
UniProt登记号 | Q9H9Z2 |
运输 | wet ice |
Gene Information | human ... LIN28A(79727) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich