产品说明

应用

Aprotinin from bovine lung has been used in insulin and glucagon-like peptide-1 assay. It has also been used a in western blotting.
抑肽酶被大量用作胰蛋白酶抑制剂。

包装

10, 25 mg in poly bottle
100, 250 mg in glass bottle

生化/生理作用

抑肽酶是一种竞争性丝氨酸蛋白酶抑制剂，可与酶的活性位点组成稳定的复合酶并同时对其进行封闭。 这种结合是可逆的，而且绝大多数抑肽酶-蛋白酶复合物可在极端的pH >10 or < 3水平时发生解离。 从结构上来说，抑肽酶是一种来自牛肺的单体球状蛋白，由58个氨基酸组成并排列在具有三个交联二硫键的单个多肽链中。
Aprotinin is a natural polypeptide of 58 amino acids. It possesses hemostatic/antiplasmin action and helps to reduce bleeding during surgeries. This prevents the necessity of blood transfusion.

单位定义

一个胰蛋白酶抑制剂单位(TIU)可将两个胰蛋白酶单位的活性降低50％，而其中一个胰蛋白酶单位在pH 7.8和25℃下可每分钟水解1.0 μ摩尔的N-α-苯甲酰基-DL-精氨酸对硝基苯胺。另一种常用的单位是KIU，而1 TIU = 1,300 KIU。
When benzoyl-L-arginine ethyl ester (BAEE) is used as substrate one BAPNA unit is approx. 45BAEE μmolar units at pH8.0 at 25℃ or approx. 9,000 BAEE A₂₅₃ units at pH7.6 at 25℃.

制备说明

This product is a lyophilized powder that has been cell culture tested. It has an activity of 3-7 TIU/mg.

基本信息

产品性质

安全信息