产品说明
一般描述
Avidin is homotetrameric protein (68kDa) obtained from egg whites and binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.
应用
Avidin-Peroxidase has been used for ELISA applications. The product can also be used for western blot applications at dilutions ranging from 1:400,000 to 1:800,000.
Western blot analysis of proteins in HUVEC cells that had underwent cell surface biotinylation were detected using HRP-conjugated avidin at 33.3 ng/ml.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.
制备说明
Affinity purified protein.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD00130587 |
| NACRES | NA.46 |
产品性质
| 质量水平 | 200 |
| 生物来源 | avidin from egg white enzyme from horseradish |
| 偶联物 | peroxidase conjugate |
| 形式 | buffered aqueous solution |
| technique(s) | indirect ELISA: 1:100,000 western blot: 1:400,000-1:800,000 using detecting β-Actin in total cell extract of HeLa cells (5-10 μg/well) |
| 运输 | dry ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
