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L-Alanine Dehydrogenase from Bacillus subtilis

品牌
Sigma-Aldrich
CAS
9029-06-5
货号
A7189
规格纯度
ammonium sulfate suspension, ≥20 units/mg protein (Lowry)
参考价格
4954.02 *本价格含增值税费
促销
服务
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  • 包邮
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数量
-+
产品名称:
9029-06-5
L-Alanine Dehydrogenase from Bacillus subtilis
L-Alanine: NAD+ oxidoreductase (deaminating)
产品介绍:

产品说明

一般描述

L-Alanine Dehydrogenase has a N-terminal substrate-binding domain and a C-terminal NAD-binding domain.

应用

L-Alanine dehydrogenase converts L-alanine to pyruvate and ammonium. L-Alanine dehydrogenase from Bacillus subtilis may be used to study enzyme inactivation and protection .
L-Alanine Dehydrogenase from Bacillus subtilis has been used in the carbon nanotube columns for H2-driven biocatalysis hydrogenation studies.

包装

250, 500 units in glass bottle

生化/生理作用

L-Alanine Dehydrogenase is essential for sporulation in Bacillus subtilis.
L-Alanine dehydrogenase is a stereospecific dehydrogenase that catalyzes the reversible deamination of L-alanine to pyruvate and ammonium. It is important for the generation of pyruvate during sporulation. L-Alanine dehydrogenase from Bacillus subtilis has a predominately ordered kinetic mechanism in which NAD binds before L-alanine. Subsequently, ammonia, pyruvate and NADH are released in that specific order. Optimal pH for the amination reaction is 8.8-9.0, whereas it is 10-10.5 for the deamination reaction. The enzyme is inactivated by divalent metal ions and p-chloromercuribenzoate, mercuric ion being most effective. The inactivation may be reversed by L- or D-cysteine.

单位定义

One unit will convert 1.0 μmole of L-alanine to pyruvate and NH3 per min at pH 10.0 at 25 ℃.

外形

Suspension in 2.4 M (NH4)2SO4 solution, pH 7.0

基本信息

酶学委员会(EC)编号1.4.1.1 ( BRENDA | IUBMB )
EC 号232-847-9
MDL编号MFCD00131441
NACRESNA.54

产品性质

质量水平200
形式ammonium sulfate suspension
specific activity≥20 units/mg protein (Lowry)
储存温度2-8℃

安全信息

象形图GHS08
警示用语:Danger
危险声明H334
预防措施声明P261 - P284 - P304 + P340 + P312 - P501
危险分类Resp. Sens. 1
储存分类代码10 - Combustible liquids
WGKWGK 3
个人防护装备Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Sigma-Aldrich

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