产品说明
一般描述
ASF1 is a histone chaperone protein that binds H3 and H4. It functions in nucleosome assembly.
Monoclonal Anti-ASF1 (mouse IgG2a isotype) is derived from the hybridoma 4A1/3 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with an ASF1A fusion protein. ASF1 (anti-silencing function 1) encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast.
免疫原
ASF1A fusion protein
应用
Monoclonal Anti-ASF1 antibody produced in mouse has been used in immunoblotting.
生化/生理作用
ASF1 (anti-silencing function 1) protein is a key component of a histone donor complex. It interacts with histones H3 and H4 and functions together with a chromatin assembly factor 1 (CAF-1) during DNA replication and repair. ASF1 also contributes to genome stability during S phase. Cells lacking ASF1 were found to be sensitive to a number of genotoxic agents that predominantly cause DNA damage by interfering with DNA replication fork progression such as hydroxyurea and camptothecin.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | 4A1/3, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~23 kDa |
| species reactivity | human, mouse |
| 浓度 | ~1.0 mg/mL |
| technique(s) | western blot: 2-4 μg/mL using whole extract of human Jurkat or Raji cells |
| 同位素/亚型 | IgG2a |
| UniProt登记号 | Q9Y294 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... ASF1A(25842) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | nwg |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
