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Anti-Axin1 (C-terminal region) antibody produced in rabbit

Anti-Axin1 (C-terminal region) 兔抗

品牌
Sigma-Aldrich
货号
A2231
规格纯度
~2.5 mg/mL, affinity isolated antibody, buffered aqueous solution
参考价格
5827.44 *本价格含增值税费
促销
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数量
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产品名称:
Anti-Axin1 (C-terminal region) antibody produced in rabbit
Anti-Axis Inhibitor 1
Anti-Axis inhibition protein 1
Anti-AXIN
Anti-Axin1 (C-terminal region) 兔抗
产品介绍:

产品说明

一般描述

Axin1 contains two conserved domains, an N-terminal regulator of G-protein signaling (RGS) domain, and a C-terminal DIX domain. The C-terminal region of axin1 is important for homodimerization, whereas the central region binds β-catenin and GSK-3b.

免疫原

synthetic peptide corresponding to amino acids 815-833 located near the C-terminal region of human axin1, conjugated to KLH. This sequence is identical in rat and mouse axin1 and highly conserved (83% identity) in human, rat, and mouse axin2.

应用

Immunohistochemistry was performed using rabbit anti-axin antibody to visualize Axin in the mouse developing cortex. Mouse brains had been previously harvested and fixed in 4% paraformaldhyde and cryoprotected in 20% sucrose. Axin was seen in the soma and axons of neurons.
Rabbit anti-axin1 (C-terminal region) antibody can be used at a concentration of 0.5-1μg/ml in western blot assays using HEK-293 cells expressing mouse axin1.

生化/生理作用

Axin1 (axis inhibitor-1 or axin) functions as a negative regulator of Wnt signaling. Axin1 is known to associate with several members of the Wnt pathway such as β-catenin, glycogen synthase kinase-3b (GSK-3b), dishevelled (Dsh) and adenomatous polyposis coli (APC).
Axin1 (axis inhibitor-1, axin) enhances the phosphorylation of β-catenin by Glycogen synthase kinase 3 β (GSK-3b). Axin1 is involved in the degradation of β-catenin by acting as a scaffold to form a complex between β-catenin, adenomatous polyposis coli (APC) and GSK-3b thus, facilitating β-catenin phosphorylation by GSK-3b. Axin1 itself is phosphorylated and its activity regulated by GSK-3b. Axin1 phosphorylation is reduced by Wnt signaling, leading to decreased affinity of interaction of axin1 and β-catenin. This results in the release of β-catenin from the degradation complex and increased stability of β-catenin in the cell. Axin1 is destabilized by Wnt signaling and its levels in the cell are reduced. Axin isoform axin2 (conductin, axil) is 44% identical to Axin1 and appears to play a similar role to Axin1 in the Wnt signaling pathway.

外形

Solution in 0.01 mM phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD02095870
NACRESNA.41

产品性质

生物来源rabbit
质量水平200
偶联物unconjugated
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
形式buffered aqueous solution
分子量antigen ~130 kDa
species reactivityrat, human, mouse
浓度~2.5 mg/mL
technique(s)immunohistochemistry: suitable
western blot: 0.5-1 μg/mL using HEK-293 cells expressing mouse axin1
UniProt登记号O15169
运输dry ice
储存温度−20℃
Gene Informationhuman ... AXIN1(8312)
mouse ... Axin1(12005)
rat ... Axin1(79257)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Sigma-Aldrich

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