产品说明
包装
25 units in glass bottle
应用
This enzyme is useful for enzymatic determination of N-acetylneuraminic acid and sialic acid when
coupled with the related enzymes in clinical analysis.
For industrial use, this enzyme is useful for enzymatic synthesis of sialic acid.
Used in the Sialic Acid Quantification Kit, SIALIC-Q
物理属性
Isoelectric point: 4.6 ± 0.1
Michaelis constant: 2.5 x 10‾3M (N-Acetylneuraminic acid)
Structure: 3 subunits (approx. 35,000) per mol of enzyme
Inhibitors: p-Chloromercuribenzoate, sodium dodecyl sulfact, Hg++, Ag+
Optimum pH: 7.5– 8.0
Optimum temp: 70℃
pH Stability: pH 6.0–9.0 (10℃, 25hr)
Thermal stability: Below 65℃ (pH 7.5, 30 min)
单位定义
One unit will release 1.0 μmole of pyruvate from NANA per min at pH 7.7 at 37 ℃.
外形
Lyophilized powder containing mannitol and EDTA
基本信息
| 酶学委员会(EC)编号 | 4.1.3.3 ( BRENDA | IUBMB ) |
| Beilstein | 2697172 |
| MDL编号 | MFCD00131656 |
| NACRES | NA.32 |
产品性质
| 形式 | lyophilized powder |
| 质量水平 | 200 |
| specific activity | ≥20 units/mg protein (biuret) |
| 分子量 | ~98 kDa |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
Sigma-Aldrich
m.cnreagent.com
