产品说明
一般描述
Most research in adipocyte biology was conducted in mouse 3T3-L1 cell lines. It has been impossible to use human adipocytes because they die within 24 hours after isolation. To facilitate the study of human adipocyte biology, primary Human Preadipocytes (HPAd) have been derived from human subcutaneous adipose tissue at various sites and adipose depot on the heart. These fibroblast-like precursor cells are cryopreserved at the end of primary culture and can be propagated two passages prior to differentiating into Human Adipocytes (HAd). Complete differentiation can be easily achieved through the use of Adipocyte Differentiation Medium. Mature HAd are expected 10 days after induction of differentiation and should remain healthy and responsive for at least 2 weeks after complete differentiation. Adipose mass can be controlled by inhibition of HPAd differentiation and increase of lypolysis. HAd are ideal cellular models for drug discovery research in the area of obesity, diabetes and cardiovascular diseases.
HPAd have been used in multiple studies investigating the cellular basis of diabetes and obesity, as well as mechanisms of action of various drug candidates. For example, it was shown that interaction between infiltrating monocytes and adipocytes affects production of metalloproteinases and osteopontin, a proinflammatory cytokine, which ultimately leads to development of more adipose tissue and insulin resistance (Samuvel, 2010, 2011). It was further demonstrated that adipocyte differentiation requires activation of Akt1 through the mTORC2-BSTA mechanism, leading to downstream suppression of FoxC2 (Yao, 2013).
细胞系来源
Adipose Tissue
应用
Drug Discovery
组分
Preadipocyte Cell Basal Medium that contains 20% FBS and 5% DMSO
制备说明
传代培养常规
Please refer to the HPAd Culture Protocol.
基本信息
NACRES | NA.81 |
产品性质
生物来源 | human adipose tissue |
质量水平 | 100 |
包装 | pkg of 500,000 cells |
生长模式 | Adherent |
核型 | 2n = 46 |
形态学 | adipocyte |
technique(s) | cell culture | mammalian: suitable |
相关疾病 | diabetes; obesity; cardiovascular diseases |
运输 | dry ice |
储存温度 | −196℃ |
安全信息
储存分类代码 | 13 - Non Combustible Solids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich