您好,欢迎来到试剂仪器网! [登录] [免费注册]
试剂仪器网
位置:首页 > 品牌 > Sigma-Aldrich > Human Preadipocytes: HPAd: Pre-Screened for Adipogenesis Signaling, adult subcutaneous

Human Preadipocytes: HPAd: Pre-Screened for Adipogenesis Signaling, adult subcutaneous

品牌
Sigma-Aldrich
货号
S802S-05A
包装型号
规格纯度
参考价格
7268.16 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Human Preadipocytes: HPAd: Pre-Screened for Adipogenesis Signaling, adult subcutaneous
产品介绍:

产品说明

一般描述

Most research in adipocyte biology was conducted in mouse 3T3-L1 cell lines. It has been impossible to use human adipocytes because they die within 24 hours after isolation. To facilitate the study of human adipocyte biology, primary Human Preadipocytes (HPAd) have been derived from human subcutaneous adipose tissue at various sites and adipose depot on the heart. These fibroblast-like precursor cells are cryopreserved at the end of primary culture and can be propagated two passages prior to differentiating into Human Adipocytes (HAd). Complete differentiation can be easily achieved through the use of Adipocyte Differentiation Medium. Mature HAd are expected 10 days after induction of differentiation and should remain healthy and responsive for at least 2 weeks after complete differentiation. Adipose mass can be controlled by inhibition of HPAd differentiation and increase of lypolysis. HAd are ideal cellular models for drug discovery research in the area of obesity, diabetes and cardiovascular diseases.

HPAd have been used in multiple studies investigating the cellular basis of diabetes and obesity, as well as mechanisms of action of various drug candidates. For example, it was shown that interaction between infiltrating monocytes and adipocytes affects production of metalloproteinases and osteopontin, a proinflammatory cytokine, which ultimately leads to development of more adipose tissue and insulin resistance (Samuvel, 2010, 2011). It was further demonstrated that adipocyte differentiation requires activation of Akt1 through the mTORC2-BSTA mechanism, leading to downstream suppression of FoxC2 (Yao, 2013).

  • Instead of protecting from hyperglycemia-induced ER stress, like it does in other cells, in adipocytes a bioactive, endogenously produced compound taurine was shown to modulate the expression of adipokines under inflammatory conditions by inhibiting the STAT-3 signaling pathway (Kim, 2013a,c), and to inhibit differentiation of preadipocytes into adipocytes (Kim, 2013b). Additionally, FGF21, which leads to reduction of body weight in animal models of obesity, was shown to act by modulating gene expression, phosphorylating Frs2a, Erk1/2, and Mypt1 (Muise, 2013) and by increasing oxidative capacity of adipocytes via AMPK–SIRT1–PGC1a cascade (Chau, 2010). Similarly, atrial natriuretic peptide (ANP) was shown to regulate lipid catabolism and reduce insulin resistance in HPAd by activating AMPK (Souza, 2011).
  • HPAd were also used to study adipocytokines in normal pregnancy and pregnancy-induced hypertension (Naruse, 2011), and to investigate the anti-obesity and hypolipidaemic properties of lotus seed extract (You, 2013, 2014). Finally, HPAd (along with Human Dermal Fibroblasts) have been used to demonstrate the role of epigenetic modifications in increasing efficiency of iPS reprogramming (Rim, 2012).

细胞系来源

Adipose Tissue

应用

Drug Discovery

组分

Preadipocyte Cell Basal Medium that contains 20% FBS and 5% DMSO

制备说明

  • 2nd passage, >500,000 cells in Preadipocyte Cell Basal Medium that contains 20% FBS and 5% DMSO
  • Can be cultured 2 passages before differentiation into Had

传代培养常规

Please refer to the HPAd Culture Protocol.

基本信息

NACRESNA.81

产品性质

生物来源human adipose tissue
质量水平100
包装pkg of 500,000 cells
生长模式Adherent
核型2n = 46
形态学adipocyte
technique(s)cell culture | mammalian: suitable
相关疾病diabetes; obesity; cardiovascular diseases
运输dry ice
储存温度−196℃

安全信息

储存分类代码13 - Non Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

推荐产品