Anti-Synapsin I Rabbit pAb

产品说明

一般描述

Recognizes the synapsin I protein in rat brain.
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the synapsin I protein.
This Anti-Synapsin I Rabbit pAb is validated for use in ELISA, Immunoblotting Immunocytochemistry Immunoprecipitation for the detection of Synapsin I.

免疫原

Bovine
purified, brovine brain synapsin I

包装

10 μg in Plastic ampoule

应用

ELISA (1:2500-1:10,000)

Frozen Sections (see application references)

Immunoblotting (1:200)

Immunocytochemistry (1:500-1:2500)

Immunoprecipitation (1 µg/200 µg lysate)

警告

Toxicity: Standard Handling (A)

外形

Lyophilized from 5 mM ammonium bicarbonate.

重悬

Reconstitute with 50 µl PBS. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

分析说明

Positive Control
Rat brain

其他说明

This antibody is specific for synapsin I and can be used to estimate synaptic density and synaptogenesis. Strong and specific immunolabeling of the synapsin I doublet seen in immunoblotting at a 1:200 dilution. This immunolabeling can be blocked by preadsorption of antibody with synapsin I. Variables associated with assay conditions will dictate the proper working dilution.
Stone, L.M., et al. 1994. J. Neurosci.14, 301.
Mandell, J.W., et al. 1992. J. Neurosci.12, 1736.
Fletcher, T.L., et al. 1991. J. Neurosci.11, 1617.
Moore, R.Y., and Berstein, M. 1989. J. Neurosci.9, 2151.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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