产品说明

一般描述

γ-Tubulin (48 kDa) is a widely expressed and highly conserved protein within the microtubule organizing centers (MTOCs) or centrosome in eukaryotic cells.
Tubulin, γ 1 is a protein encoded by the TUBG1 gene in humans. This gene encodes a member of the tubulin superfamily. γ-tubulin interacts with Arp2/3, a complex that nucleates actin filaments and with the Arp2/3 activator WASH. It is found to have relationship with actin filament dynamics.

免疫原

对应人 γ-微管蛋白(氨基酸 38-53，C 端添加赖氨酸) N 端区域的合成肽与 KLH 结合。该序列对 γ-微管蛋白具有特异性，而对微管蛋白家族的其他成员如 α−β−, δ和 ε−微管蛋白。该序列在小鼠和大鼠的 γ-微管蛋白中相同，并在物种( 果蝇 、 曲霉 和酵母 γ-微管蛋白)中高度保留。

应用

Anti-γ- Tubulin-Cy3 antibody produced in rabbit has been used in direct immunofluorescence (IF)and immunofluorescent staining.
Anti-γ-Tubulin-Cy3^™ antibody produced in rabbit is suitable for direct immunofluorescence at a dilution of 1:200 using methanol/acetone-fixed chicken fibroblats cells or HeLa cells (human).

生化/生理作用

γ-Tubulin binds the microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome.
γ-Tubulins in the γ-TuRC might promote microtubule nucleation by providing a template that enhances the intrinsically weak lateral interaction between αβ-tubulin heterodimers when associated laterally. It has a unique functional property of a monomeric tubulin. γ-tubulin is also found to be expressed in lung cancer. Cep70 as an important centrosomal protein that interacts with γ-tubulin and plays a critical role in the regulation of mitotic spindle assembly.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

法律信息

Cy is distributed under license from Amersham Biosciences Limited.
Cy3 is a trademark of Cytiva

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

安全信息