产品说明
一般描述
Anti-Green Fluorescent Protein (26-39), rabbit polyclonal, recognizes the ~28 kDa GFP and some GFP fusion proteins. It is validated for use in Western blotting and immunocytochemistry.
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~28 kDa GFP and some GFP fusion proteins.
Recognizes the ~28 kDa GFP and some GFP fusion proteins.
免疫原
Aequora victoria
a synthetic peptide corresponding to amino acids 26-39 of Aequorea victoria wild-type green fluorescent protein
包装
100 μL in Plastic ampoule
Please refer to vial label for lot-specific concentration.
应用
Immunoblotting (1:2000)
Immunocytochemistry (1:200)
警告
Toxicity: Standard Handling (A)
外形
In PBS, 1 mg/ml BSA.
重悬
Following initial thaw, aliquot and freeze (-20°C).
其他说明
This sequence is completely conserved between GFP and the modified and enhanced Blue, Green, and Yellow FP variants. This antibody has been successfully used in immunoblotting and immunocytochemistry protocols to detect GFP and fusion proteins. It detects a ~28 kDa protein that corresponds to GFP in immunoblots of protein extract from cells transfected with, and overexpressing, GFP isoforms. Antibody should be titrated for optimal results in individual sytems.
Cubitt, A.B., et al. 1995. Trends Biochem. Sci.20, 448.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | liquid |
| 包含 | ≤0.1% sodium azide as preservative |
| manufacturer/tradename | Calbiochem® |
| 储存条件 | OK to freeze avoid repeated freeze/thaw cycles |
| 同位素/亚型 | IgG |
| 运输 | wet ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
