Anti-α-Tubulin Mouse mAb (DM1A)

产品说明

一般描述

This Anti-α-Tubulin Mouse mAb (DM1A) is validated for use in Immunoblotting, Immunofluorescence for the detection of α-Tubulin.
Purified mouse monoclonal antibody generated by immunizing Balb/c57 mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~60 kDa tubulin protein.
Recognizes the ~60 kDa tubulin protein found in all eukaryotic cells. May be used as a positive control.

免疫原

Chicken
native chick brain microtubules

应用

Immunoblotting (20-100 ng/ml, colorimetric or chemiluminescence; see application references)

Immunofluorescence (5 µg/ml)

包装

Please refer to vial label for lot-specific concentration.

警告

Toxicity: Standard Handling (A)

外形

In 50 mM sodium phosphate buffer, 0.2% gelatin.

分析说明

Positive Control
Any cell line
Negative Control
trp E (Ab-1)

其他说明

Tubulin is found in all cells and is used as a positive control for immunofluorescence or immunoblotting. Antibody should be titrated for optimal results in individual systems.
Blose, S.H., et al. 1984. J. Cell. Biol.98, 847.
Lin, J.J.C., et al. 1984. In: Monoclonal antibodies and functional cell lines. Kennett, R.H., Bechtol, K.B. and McKearn, T.J., eds. Plenum Publishing Corp.
Lin, J.J.C. 1982. Cold Spring Harbor Symp. on Quant. Biol.46, 769.
Goldman, R.D., et al. 1979. Ann. Rev. Physiol.41, 701.
Goldman, R., Pollard, T. and Rosenbaum, J. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C, Cold Spring Harbor, New York.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

安全信息