产品说明
应用
Biotin-dextran is used in tissue staining to prepare neurons for detection by avidin-conjugated detection systems. It is taken up by neuronal processes and transported bi-directionally. It infiltrates axon boutons in the anterograde direction and dendritic processes in the retrograde direction. Staining can be observed in fixed and sectioned tissue from two days to two weeks after biotin-dextran is injected into the brain or spinal cord. It can also be applied to cut nerve tracts. Biotinylated neurons are detected by either light or electron microscopy following incubation with avidin-horseradish peroxidase conjugate and the electron-dense substrate 3,3′-diaminobenzidine (DAB).
包装
10, 25 mg in poly bottle
基本信息
MDL编号 | MFCD00130657 |
eCl@ss | 34058011 |
NACRES | NA.25 |
产品性质
偶联物 | biotin conjugate |
质量水平 | 200 |
形式 | powder |
分子量 | 10,000 |
标记范围 | ~2 mol per mol |
溶解性 | H2O: soluble 50 mg/mL, clear, colorless to faintly yellow |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 13 - Non Combustible Solids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
个人防护装备 | Eyeshields, Gloves, type N95 (US) |
Sigma-Aldrich