产品说明
一般描述
Red-Gal is a chromogenic substrate for b-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Red-Gal is designed to replace X-Gal in the blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Red-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Red-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
应用
Magenta-Gal has been used for staining synthetic cytoplasmic or nuclear LacZ mRNA. It has also been used to detect the expression of the EphB1-β-gal fusion protein. ,Magenta-Gal is suitable for use in the selection of recombinant bacterial colonies with the lac+ phenotype.
Chromogenic substrate for β-galactosidase producing a red or magenta insoluble product.
原理
When Red-Gal is hydrolyzed by β-galactosidase, the resulting product will form a red precipitate. Lac+ colonies grown in the presence of Red-Gal turn an intense red color, allowing for easy differentiation between lac+ and lac- colonies.
基本信息
| 经验(实验)分子式 | C14H15BrClNO6 |
| 分子量 | 408.63 |
| MDL编号 | MFCD02683897 |
| PubChem化学物质编号 | 329773145 |
| NACRES | NA.85 |
产品性质
| 质量水平 | 200 |
| 等级 | for molecular biology |
| 无菌性 | non-sterile |
| 产品线 | BioReagent |
| 测定 | ≥98.0% (HPLC) |
| 形式 | powder |
| 溶解性 | methanol: soluble |
| 适用性 | suitable for substrate for β-galactosidase |
| 储存温度 | −20℃ |
| SMILES string | OC[C@H]1O[C@H](Oc2c[nH]c3cc(Cl)c(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O |
| InChI | 1S/C14H15BrClNO6/c15-6-1-5-8(2-7(6)16)17-3-9(5)22-14-13(21)12(20)11(19)10(4-18)23-14/h1-3,10-14,17-21H,4H2/t10-,11+,12+,13-,14+/m1/s1 |
| InChI key | CHRVKCMQIZYLNM-HTOAHKCRSA-N |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
Sigma-Aldrich
m.cnreagent.com
