产品说明
一般描述
Cell replacement techniques such as stem-cell or fetal-cell therapies show great promise for many diseases. Cross-species grafting between human and animal cells or tissues requires the identification and localization of the donor material in the host environment. Antibodies to human-specific nuclei are useful cytological and histological markers for identifying human cells in xenograft models. MAB1281 stains nuclei of all human and primate cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. The antibody does not react with nuclei from mouse or rat.
特异性
This antibody recognizes human and primate nuclei. Antibody is not reactive with rat or mouse nuclei.
免疫原
Human nuclei
应用
Evaluated by Immunocytochemistry in human bone marrow mesenchymal stem cells (SCR108). Immunocytochemistry Analysis: A 1:200 dilution of this antibody detected nuclei in human bone marrow mesenchymal stem cells (SCR108). Evaluated by Immunocytochemistry in primary mouse embryonic fibroblasts (PMEFs). Immunocytochemistry Analysis: A 1:200 dilution of this antibody did not detect nuclei in primary mouse embryonic fibroblasts (PMEFs).
Detect Nuclei Marker using this mouse monoclonal antibody, Anti-Human Nuclei Antibody, clone 235-1 & Biotin Conjugate validated for use in ICC.
Research Category
Stem Cell Research
Neuroscience
Research Sub Category
Neuroregenerative Medicine
Neural Stem Cell Migration
质量
Evaluated by Immunocytochemistry in A431 human epidermoid cancer cells. Immunocytochemistry Analysis: A 1:200 dilution of this antibody detected nuclei in A431 human epidermoid cancer cells.
外形
Purified mouse monoclonal IgG1 conjugated to Biotin in PBS with 0.1% sodium azide and 15mg/ml BSA.
Protein G Purified
储存及稳定性
Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.
分析说明
Control
A431 human epidermoid cancer cells.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
偶联物 | biotin conjugate |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | 235-1, monoclonal |
species reactivity | human |
technique(s) | immunocytochemistry: suitable |
同位素/亚型 | IgG1 |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich