产品说明
特异性
Recognizes Thr567 phosphorylated ezrin, Thr564 phosphorylated radixin, and Thr558 phosphorylated moesin. Antibody does not cross-react with other related phospho proteins such as merlin or band 4.1.
免疫原
KLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Thr567 of human ezrin
Epitope: phospho-specific
应用
Research Category
Cell Structure
Anti-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody, phospho-specific detects level of Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) & has been published & validated for use in IH(P) & WB.
Western blotting 1:1,000 (for best results, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight). The antibody recognizes 75KDa (Moesin, and ~80kDa Ezrln & Radixin) bands.
Immunohistochemistry (paraffin): 1:50
Optimal working dilutions must be determined by the end user.
Research Sub Category
Cytoskeleton
目标描述
75KDa Moesin, and ~80kDa Ezrin & Radixin
外形
ImmunoAffinity Purified
Liquid in 10 mM sodium HEPES, pH 7.5, 150 mM NaCl, 100 μg/ml BSA, with 50% glycerol.
储存及稳定性
Maintain at -20°C for 12 months, do not aliquot.
分析说明
Control
Cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | affinity isolated antibody |
antibody product type | primary antibodies |
克隆 | polyclonal |
纯化方式 | affinity chromatography |
species reactivity | monkey, mouse, rat, human |
manufacturer/tradename | Chemicon® |
technique(s) | immunohistochemistry: suitable (paraffin) western blot: suitable |
NCBI登记号 | NM_004252.2 |
UniProt登记号 | O14745 |
运输 | dry ice |
Gene Information | human ... SLC9A3R1(9368) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |
Sigma-Aldrich