产品说明
应用
Clostripain from Clostridium histolyticum has been used as a proteolytic enzyme in perfusate to detect its effect on tube hematocrit. It has also been used in limited proteolysis of DNA polymerase (gp43) of phage T4 (RB69 gp43).
生化/生理作用
Clostripain from Clostridium histolyticum is composed of two polypeptide chains, with molecular masses of 41.7 kDa and 15.4 kDa. Clostripain has a highly restricted substrate specificity for Arg-Xaa peptide bonds. Therefore, clostripain has been explored as a potential enzyme for protein sequencing purposes. It has also been studied as a catalyst for condensation of pharmaceutically important peptides containing Arg-Pro bonds.
包装
Bottomless glass bottle. Contents are inside inserted fused cone.
质量
Purified, essentially salt-free
单位定义
One unit will hydrolyze 1.0 μmole of BAEE per min at pH 7.6 at 25 ℃ in the presence of 2.5 mM DTT.
分析说明
The enzyme must be activated for 2-3 hours before use by dissolving in 2.5 mM dithiothreitol containing 1.0 mM calcium acetate.
基本信息
| 酶学委员会(EC)编号 | 3.4.22.8 ( BRENDA | IUBMB ) |
| EC 号 | 232-822-2 |
| MDL编号 | MFCD00130814 |
| NACRES | NA.54 |
产品性质
| 形式 | lyophilized powder |
| 质量水平 | 100 |
| specific activity | ≥20 units/mg solid |
| 分子量 | 15.4 kDa 41.7 kDa |
| 杂质 | salt, essentially free |
| 储存温度 | 2-8℃ |
安全信息
| 象形图 |   |
| 警示用语： | Danger |
| 危险声明 | H315 - H319 - H334 - H335 |
| 预防措施声明 | P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 |
| 危险分类 | Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3 |
| 靶器官 | Respiratory system |
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
Sigma-Aldrich
m.cnreagent.com
