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Anti-phospho-Cofilin (pSer3) antibody produced in rabbit

Monoclonal Ant-phospho-Cofilin (pSer3) 兔抗

品牌
Sigma-Aldrich
货号
C8992
规格纯度
IgG fraction of antiserum, buffered aqueous solution
参考价格
6560.33 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-phospho-Cofilin (pSer3) antibody produced in rabbit
Anti-CFL1
Anti-18 kDa phosphoprotein
Anti-p18
Anti-cofilin 1 (non-muscle)
Monoclonal Ant-phospho-Cofilin (pSer3) 兔抗
产品介绍:

产品说明

一般描述

Cofilin is a small phosphoinositide-sensitive actin-binding protein capable of depolymerizing actin-filaments in vitro. It has gelsolin-like actin filament-severing protein similar to destrin/ADF (Actin Depolymerizing Factor). Cofilin has has a short β-strand at the C terminus. In mammals it has two isoforms: non-muscle (NM-CF, CF-L1) and muscle (M-CF, CF-L2). The protein is ubiquitiously present in tissues of eukaryotes and is especially abundant in neuronal tissues. It can shuttle between the cytoplasm and the nucleus in response to various stresses or signals, and may translocate from the cytoplasm to the plasma membrane in various cells.

免疫原

synthetic phosphopeptide corresponding to amino acids 2-9 (pSer3) of human cofilin with a C-terminal added cysteine, conjugated to KLH. This sequence is identical in mouse, rat, and pig.

应用

Anti-phospho-Cofilin antibody was used:

  • as primary antibody for immunoblottingto to study the role of the Rac1 GTPase.
  • for western blot analysis in a comparative study of signals induced by integrin ligation during cell attachment, mechanical force from intracellular contraction, or cell stretching by external force.
  • as primary antibody for western blotting in a study to detect the level of cofilin.

生化/生理作用

Cofilin binds stoichiometrically to monomeric G-actin and actin protomers in filaments in an apparently pH-dependent, Ca2+ independent manner. Cofilin intercalates between longitudinally associated actin monomers within the filament and distorts its helical twist.It cleaves the filaments and accelerates actin subunits dissociation from their ‘pointed′ ends under specific conditions. Cofilin is essential for viability and is important for many cellular processes involving actin remodeling such as motility at the leading edge of cells, polarized cell growth, endocytosis, phagocytosis, cellular activation, cytokinesis, and pathogen intracellular motility. It′s activity is regulated through reversible phosphorylation and dephosphorylation. In phosphorylated form, it behaves inactive and unable to bond with actin. Phosphorylation of cofilin is regulated in vertebrates by at least four protein kinases: LIM Kinase 1, LIM Kinase 2, Testicular Kinase 1, and Testicular Kinase 2. The dephosphorylation of cofilin enables its actin severing and depolymerizing activity and drives directional cell motility, thus providing a simple phosphoregulatory mechanism for actin reorganization.

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD04040757
NACRESNA.41

产品性质

生物来源rabbit
质量水平200
偶联物unconjugated
抗体形式IgG fraction of antiserum
antibody product typeprimary antibodies
克隆polyclonal
形式buffered aqueous solution
分子量antigen ~19 kDa
species reactivitymouse, rat (predicted), pig (predicted), human
technique(s)western blot: 1:2,000-1:4,000 using whole extracts of HeLa human epithelioid carcinoma and mouse NIH3T3 cells
UniProt登记号P23528
运输dry ice
储存温度−20℃
Gene Informationhuman ... CFL1(1072)
mouse ... Cfl1(12631)
rat ... Cfl1(29271)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Sigma-Aldrich

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