产品说明
一般描述
Cystatin B is a member of the type 1 (stefins) superfamily of cystatins. Cystatin B is encoded by the CSTB gene and functions as an intracellular thiol protease inhibitor regulating the activity of members of the papain family and cathepsins B, H, and L. Cystatin B may also protect cellular components from the effects of proteases that leak from lysosomes. Mutations in the CSTB gene are thought to be the primary defect in patients with Progressive Myoclonus Epilepsy (EPM1).
应用
Suitable for use as a cysteine-protease inhibitor. Is tested for suitability as an inhibitor of the enzymatic activity of papain.
生化/生理作用
Cystatin B was shown to interact with the protein RACK-1, β-spectrin and neurofilament light chain NF-L forming a unique multiprotein complex specifically in the cerebellum, confirming its function in the brain.
重悬
Cystatin B should be reconstituted at 100 μg/mL in steile 25 mM Tris pH 7.5 and 100 mM NaCl. Reconstituted product should be stored in single-use aliquots at -70° C and should be stable for at least 3 months.
基本信息
| NACRES | NA.77 |
产品性质
| 质量水平 | 200 |
| 重组 | expressed in E. coli |
| 描述 | contains a N-terminal 7X His-tag |
| 测定 | ≥95% (SDS-PAGE) |
| 形式 | solid |
| 分子量 | apparent mol wt ~13 kDa by SDS-PAGE (reducing) |
| NCBI登记号 | NP_000091 |
| 储存温度 | −20℃ |
| Gene Information | human ... CSTB(1476) |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
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