产品说明
生化/生理作用
Ubiquitous serine-threonine kinase that phosphorylates a broad spectrum of substrates, and regulates many cellular processes. The catalytic subunit is released following binding of cyclic AMP to the regulatory subunits of the PKA holoenzyme. The free catalytic subunit has intrinsic activity and does not require added cyclic AMP.
外形
Solution in 30 mM potassium phosphate buffer, pH 7.4, containing 50% glycerol, 150 mM KCl, 1 mM EDTA, and 1 mM DTT.
分析说明
One unit will transfer 1 nanomole of phosphate from 32P-ATP to kemptide substrate per minute at pH 7.4 at 30 ℃ (measured by a radioactive filter-binding assay).
Km (ATP) = 25 μM at 30 ℃, pH 7.4. Km (kemptide) = 42 μM (33 μg/ml) at 30 ℃, pH 7.4.
基本信息
| MDL编号 | MFCD03458215 |
| NACRES | NA.32 |
产品性质
| 质量水平 | 200 |
| 重组 | expressed in E. coli |
| 测定 | >90% (SDS-PAGE) |
| 形式 | buffered aqueous glycerol solution |
| specific activity | >1000 units/mg protein |
| 分子量 | 43.5 kDa |
| UniProt登记号 | P17612 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... PRKACA(5566) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
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