产品说明
生化/生理作用
Useful in screening caspase inhibitors, studying enzyme regulation, determining specificity of caspase substrates, or as a positive control in caspase activity assays.
Cleaves the substrates consisting of consensus sequence VEID.
Spontaneously undergoes autoprocessing to yield the subunits associated to form the active enzyme.
单位定义
One unit will hydrolyze 1 nmol of the caspase substrate VEID-pNA to VEID and p-nitroaniline per hour at pH 7.2 at 37 ℃.
外形
Contains 0.052% ammonium sulfate, 0.158% Tris−HCl, 0.76% sodium chloride.
基本信息
NACRES | NA.32 |
产品性质
质量水平 | 100 |
重组 | expressed in E. coli |
测定 | >90% (SDS-PAGE) |
形式 | lyophilized powder |
specific activity | 13,000 units/mg protein |
分子量 | ~30 kDa |
溶解性 | PBS: soluble |
运输 | dry ice |
储存温度 | −70℃ |
Sigma-Aldrich