产品说明
应用
Cholesterol oxidase is used to determine serum cholesterol. The enzyme also finds application in the microanalysis of steroids in food samples and in distinguishing 3-ketosteroids from 3b-hydroxysteroids. Transgenic plants expressing cholesterol oxidase are being investigated in the fight against the cotton boll weevil. CHOD has also been used as a molecular probe to elucidate cellular membrane structures.
包装
100 units in glass bottle
生化/生理作用
Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step. This is subsequently isomerized to cholest-4-en-3-one with the release of H2O2. Optimum pH of the enzyme is 7.0. Hg2+, Ag+, ionic detergents inhibit the enzyme activity.
单位定义
One unit will convert 1.0 μmol of cholesterol to 4-cholesten-3-one per minute at 37 ℃ and pH 7.0 in a peroxidase linked system.
制备说明
Dissolves in cold 50 mM potassium phosphate buffer, pH 7.0. Solution is to be prepared just before use.
基本信息
| 酶学委员会(EC)编号 | 1.1.3.6 ( BRENDA | IUBMB ) |
| EC 号 | 232-842-1 |
| MDL编号 | MFCD00130783 |
| NACRES | NA.54 |
产品性质
| 质量水平 | 200 |
| 形式 | lyophilized powder |
| specific activity | ≥10 units/mg protein |
| 分子量 | 55 kDa |
| 溶解性 | 50 mM potassium phosphate buffer, pH 7.0: soluble |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
Sigma-Aldrich
m.cnreagent.com
