产品说明
一般描述
The ApopTag
The ApopTag
应用
INTRODUCTION
ApopTag
Principles of the Procedure
The reagents provided in all ApopTag
DNA fragments which have been labeled with the digoxigenin-nucleotide are then allowed to bind an anti-digoxigenin antibody that is conjugated to fluorescein (Figure 1A). Fluorescent antibodies provide sensitive detection in immunohistochemistry or immunocytochemistry (i.e. on tissue or cells) and are not subject to experimental variations due to the substrate or the development step. This mixed molecular biological-histochemical systems allows for sensitive and specific staining of very high concentrations of 3′-OH ends that are localized in apoptotic bodies.
The ApopTag
The ApopTag Plus Fluorescein In Situ Apoptosis Detection Kit detects apoptotic cells in situ by the indirect TUNEL method, utilizing an anti-digoxigenin antibody that is conjugated to a fluorescein reporter molecule.
组分
Equilibration Buffer 90416 3.0 mL -15°C to -25°C
Reaction Buffer 90417 2.0 mL -15°C to -25°C
TdT Enzyme 90418 0.64 mL -15°C to -25°C
Stop/Wash Buffer 90419 20 mL -15°C to -25°C
Blocking Solution 90425 2.6 mL -15°C to -25°C
Anti-Digoxigenin-Fluorescein* 90426 2.1 mL 2°C to 8°C
Plastic Coverslips 90421 100 ea. Room Temp.
Control Slides 90422 2 ea. Room Temp.
*affinity purified sheep polyclonal antibody
储存及稳定性
1. Store the kit at -15°C to -25°C until the first use. After the first use, if the kit will be used within three months, store the TdT Enzyme (#90418) at -15°C to -25°C and store the remaining components at 2°C to 8°C.
2. Protect the anti-digoxigenin fluorescein antibody (#90426) from unnecessary exposure to light.
Precautions
1. The following kit components contain potassium cacodylate (dimethylarsinic acid) as a buffer: Equilibration Buffer (#90416), Reaction Buffer (#90417), and TdT Enzyme (#90418). These components are harmful if swallowed; avoid contact with skin and eyes (wear gloves, glasses) and wash areas of contact immediately.
2. Antibody Conjugates (#90426) and Blocking Solutions (#90425) contain 0.08% sodium azide as a preservative.
3. TdT Enzyme (#90418) contains glycerol and will not freeze at -20°C. For maximum shelf life, do not warm this reagent to room temperature before dispensing.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32161000 |
产品性质
质量水平 | 100 |
manufacturer/tradename | ApopTag Chemicon® |
technique(s) | flow cytometry: suitable immunocytochemistry: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
detection method | fluorometric |
运输 | dry ice |
安全信息
储存分类代码 | 10 - Combustible liquids |
Sigma-Aldrich