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Diaphorase from Clostridium kluyveri

硫辛酰胺脱氢酶

品牌
Sigma-Aldrich
货号
D2197
规格纯度
recombinant, expressed in E. coli, Animal-component free
参考价格
936.43 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Diaphorase from Clostridium kluyveri
Lipoamide Dehydrogenase
Diaphorase
Lipoyl Dehydrogenase
硫辛酰胺脱氢酶
产品介绍:

产品说明

应用

Diaphorase from Clostridium kluyveri has been used in lactate dehydrogenase assay in gill cells and impregnated on the strip for formate dehydrogenase assay.
Diaphorase is used in various assays to oxidate β-NADH or β-NADPH in the presence of an electron acceptor. Diaphorase from Clostridium Kluyveri has been used for semiquinone generation during benzoquinone reduction. Product D2197 is animal component free.

包装

100, 300, 500 units in poly bottle

生化/生理作用

Diaphorase catalyzes the oxidation of either β-NADH or β-NADPH in the presence of an electron acceptor such as methylene blue or 2,6-dichlorophenolindophenol. Diaphorases which are specific for either β-NADH or β-NADPH are known. The pig heart enzyme of Straub seems to have native diaphorase (β-NADH specific) as well as lipoic and lipoamide dehydrogenase activities. It is reported to be a single protein. However, Massey reports that diaphorase is probably a denatured lipoamide dehydrogenase. Pre-incubation of the pig heart preparation with Cu2+ reduces the lipoamide dehydrogenase activity and proportionately increases the β-NADH diaphorase activity. In our laboratory, we have demonstrated this copper effect to some degree on the pig heart enzyme, but no appreciable effect was observed on the Clostridium kluyveri or torula yeast preparations. The lipoamide dehydrogenase:diaphorase ratio is a measure of the denaturation.

单位定义

One unit of diaphorase will oxidize 1.0 micromole of beta-NADH per minute at pH 7.5 at 25 degrees C, with the corresponding reduction of the appropriate electron acceptor.

外形

Supplied as a lyophilized powder containing trehalose.

基本信息

酶学委员会(EC)编号1.8.1.4 ( BRENDA | IUBMB )
NACRESNA.54

产品性质

质量水平200
重组expressed in E. coli
形式solid
specific activity>=30 units/mg protein (biuret)
储存温度−20℃

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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