产品说明
一般描述
E2F6 transcription factor belongs to the third class of E2Fs. The E2F transcription factors are a family of genes that play critical roles in regulation of cellular proliferation and differentiation.
免疫原
recombinant human E2F6 protein
应用
Monoclonal Anti-E2F6 antibody produced in mouse is suitable for immunocytochemistry and indirect ELISA. It is also suitable for western blotting at a working concentration of 2-4μg/mL using HeLa nuclear cell extract.
生化/生理作用
The third class of E2Fs lacks both the retinoblastoma protein binding and transcriptional activation domain, and is an active repressor of transcription. The activity of E2F6 is mediated through its recruitment of polycomb transcriptional repressor complexes in a histone methylation independent manner. E2F6 associates with E2F target genes that are activated at G1/S phase. Its repression activity during the S-phase distinguishes G1/S and G2/M transcription during cell cycle. It may be involved in various cancerous processes.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | TFE61, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~35 kDa |
| species reactivity | human |
| 浓度 | ~1.0 mg/mL |
| technique(s) | immunocytochemistry: suitable indirect ELISA: suitable western blot: 2-4 μg/mL using HeLa nuclear cell extract |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | O75461 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... E2F6(1876) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
Sigma-Aldrich
m.cnreagent.com
