Enterobactin

产品名称：

28384-96-5
Enterobactin
Enterochelin

产品介绍：

产品说明

一般描述

Enterobactin synthesis occurs via nonribosomal peptide synthetases. The precursor chorismic acid is converted to intermediates isochorismate, 2,3-dihydro-2,3-dihydroxybenzoate and 2,3-dihydroxybenzoic acid (DHB). Final step involves the DHB and L-serine amide linkage. A total of six enzymes (ent A −F) mediate enterobactin biosynthesis.

应用

Enterobactin has been used:

as a positive control in tryptophan fluorescence quenching experiments
as a reference standard in high performance liquid chromatography to quantify Kosakonia radicincitans culture medium siderophores
in the crystallization reservoir to remove iron contamination in the medium and in Fe-enterbactin binding studies

This preparation of enterobactin is not bound to iron and will bind to Fe³⁺ and Fe²⁺ in solution.

生化/生理作用

Iron mobilization and uptake by microbes is mediated by low molecular weight complexing agents named siderophores. Enterobactin is a catechol (a benzenediol, C₆H₄(OH)₂) type siderophore produced in small quantities by Escherichia coli and related enteric bacteria when grown on iron deficient media, and is one of the most powerful ferric ion complexing agents known. Enterobactin is a very effective sequestering agent for iron, able to remove iron from proteins, insoluble iron complexes, and other siderophores. Studies of the chemistry, regulation, synthesis, recognition, and transport of enterobactin make it the best-understood siderophore.

基本信息

经验(实验)分子式	C₃₀H₂₇N₃O₁₅
分子量	669.55
NACRES	NA.25

产品性质

生物来源	Escherichia coli
质量水平	200
测定	≥98% (HPLC)
运输	wet ice
储存温度	−20℃
InChI	1S/C30H27N3O15/c34-19-7-1-4-13(22(19)37)25(40)31-16-10-46-29(44)18(33-27(42)15-6-3-9-21(36)24(15)39)12-48-30(45)17(11-47-28(16)43)32-26(41)14-5-2-8-20(35)23(14)38/h1-9,16-18,34-39H,10-12H2,(H,31,40)(H,32,41)(H,33,42)/t16-,17-,18-/m0/s1
InChI key	SERBHKJMVBATSJ-BZSNNMDCSA-N

安全信息

储存分类代码	11 - Combustible Solids
闪点(F)	Not applicable
闪点(C)	Not applicable