产品说明

一般描述

表皮生长因子(EGF)是小的促有丝分裂多肽(∼6 kDa)，存在于许多哺乳动物物种中，分布在许多组织和体液中。四种ErbB (HER) 家族受体酪氨酸激酶(包括EGFR/ErbB1、ErbB2、 ErbB3和 ErbB4)介导针对EGF家族成员的反应。人类和小鼠的EGF非常相似，但是它们的物理和化学性质并不相同。在构成两种多肽中每一种多肽的53个氨基酸残基中，有37个是两种分子共有的，并且在相同的相对位置上形成三个二硫键。

应用

来自小鼠的表皮生长因子已用作基础(N2B27)培养基补充剂，用于培养分化的小鼠胚胎干(E14)细胞。

生化/生理作用

EGF(表皮生长因子)可参与细胞增殖、分化和存活。 此外，它还可影响各种生物活动，如血管生成、抑制胃酸分泌、调节多种激素的合成、细胞外基质蛋白质的合成和转换、骨组织钙释放(从而促进骨吸收)、成纤维细胞和上皮细胞的化学趋化作用、单独或与其他细胞因子组合介导伤口愈合过程。 EGF对于多种细胞类型具有促有丝分裂作用，包括成纤维细胞、上皮细胞、内皮细胞、软骨细胞、以及SV40-3T3细胞。

外形

该产品从磷酸盐缓冲盐水(pH 7.4，0.2 μm滤膜过滤)中冻干。

引用

1. Carpenter, G., and Cohen, S., Epidermal growth factor. Annu. Rev. Biochem., 48, 193-216 (1979).
2. Jorissen, R.N., et al., Epidermal growth factor: mechanisms of activation and signaling. Exp. Cell Res., 284, 31-53 (2003).
3. Herbst, R.S., Review of epidermal growth factor receptor biology. Int.J. Radiat. Oncol. Biol., Phys., 59, S21-S26 (2004).
4. Mehta, V.B., and Besner, G.E., HB-EGF promotes angiogenesis in endothelial cells via PI3-kinase and MAPK signaling pathways. Growth Factors, 25, 253-263 (2007).
5. Bower, J.M., et al., The inhibition of gastric acid secretion by epidermal growth factor. Experientia, 31, 825-826 (1975).
6. Schonbrunn, A., et.al., Epidermal growth factor and thyrotropin-releasing hormone act similarly on a clonal pituitary cell strain. Modulation of hormone production and inhibition of cell proliferation. J. Cell Biol., 85, 786-797 (1980).
7. Mimura, Y., et al., Epidermal growth factor induces fibronectin expression in human dermal fibroblasts via protein kinase C delta signaling pathway. J. Invest. Dermatol., 122, 1390-1398 (2004).
8. Warner, M.R. et al., Ametantrone inhibits prostaglandin-mediated resorption in bone organ culture. Prostaglandins, 28, 469-476 (1984).
9. Grotendorst, G.R., et al., EGF and TGF-alpha are potent chemoattractants for endothelial cells and EGF-like peptides are present at sites of tissue regeneration. J. Cell. Physiol., 139, 617623 (1989).
10. Schultz, G., et al., EGF and TGF-alpha in wound healing and repair. J. Cell. Biochem., 45, 346-352 (1991).
11. George-Nascimento, C., et al., Characterization of recombinant human epidermal growth factor produced in yeast. Biochemistry, 27, 797-802 (1988).

基本信息

产品性质

安全信息