pBICEP-CMV^TM-3 Expression Vector

产品说明

一般描述

The pBICEP-CMV-3 bicistronic expression vector is a 5.3 kb derivative of pCMV5 used for transient or stable co-expression of an N-terminal 1´ FLAGÒ fusion protein and a selection gene in mammalian cells.

包装

20 μg in poly bottle

应用

For transient, cytoplasmic expression of an N-terminal FLAG^® fusion and a second gene of interest or selection marker from bicistronic mRNA. Two genes can be cloned into MCS1 and MCS2 for transcription of a single message from the CMV promoter. This vector is useful for protein-protein interaction studies, multi-subunit proteins, and cloning a selection marker of choice.

生化/生理作用

The presence of two multiple cloning sites not only allows the user to clone a target gene, but a selectable marker of their choice, as well. The promoter-regulatory region of the human cytomegalovirus immediate early promoter drives transcription of the FLAG-fusion construct along with a downstream selection gene. The EMCV IRES region controls translation of the selection gene by recruiting the ribosomal subunits for cap-independent translational initiation. Depending on the mode of selection, stable transfectants can be generated by transfection using the appropriate ESCORTä product line specific for the cell type.

其他说明

您可在我们的FLAG^®文献门户上浏览更多应用文献。

法律信息

FLAG is a registered trademark of sigma-aldrich Co. LLC
pBICEP-CMV is a trademark of sigma-aldrich Co. LLC

产品性质

安全信息