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Fpg Protein from Escherichia coli

品牌
Sigma-Aldrich
CAS
78783-53-6
货号
F3174
规格纯度
≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution, >20,000 units/mg protein
参考价格
8503.71 *本价格含增值税费
促销
服务
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数量
-+
产品名称:
78783-53-6
Fpg Protein from Escherichia coli
Fapy DNA glycosylase
Formamidopyrimidine DNA glycosylase
MutM
产品介绍:

产品说明

一般描述

Fpg contains two domains separated by a flexible hinge.
Formamidopyrimidine-DNA glycosylase (Fpg) is a DNA repair enzyme found in Escherichia coli, which contains one zinc atom. Proximal to its C-terminal, it contains a zinc-finger motif of CC/CC type.

应用

Fpg Protein from Escherichia coli has been used for the assessment of DNA oxidative damage using comet assay.

包装

10 μg in poly bottle

生化/生理作用

Fpg is a key enzyme in the DNA base excision repair pathway (BER). It catalyses the excision of a broad spectrum of modified purines. Fpg has both DNA glycosylase activity that removes the mutated base and AP-lyase activity that releases ribose leaving both 5′- and 3′-phosphorylated ends in the DNA. The zinc finger motif at its C-terminus is responsible for the DNA binding and AP-lyase activity. In addition, its N-terminal proline acts as a nucleophile to produce a Schiff base intermediate that is essential for enzyme action.
Fpg specifically acts on 3′- and 5′-phosphodiester bonds.
Formamidopyrimidine-DNA glycosylase (Fpg) cleaves double-stranded DNA containing the damaged base 8-oxo-7,8-dihydroguanine. It functions as an N-glycosylase and apurinic/apyrimidinic lyase.

单位定义

One unit will cleave 50% of 0.5 pmol of double-stranded DNA oligomer substrate (8-oxoguanine−mutated) in 10 min at 25 ℃.

外形

Solution in 50% glycerol containing 50 mM potassium HEPES, pH 7.5, 1 mM DTT, 1 mM EDTA, and 200 mM NaCl.

基本信息

酶学委员会(EC)编号3.2.2.23 ( BRENDA | IUBMB )
MDL编号MFCD02264758
NACRESNA.32

产品性质

质量水平200
生物来源Escherichia coli
重组expressed in E. coli
测定≥90% (SDS-PAGE)
形式buffered aqueous glycerol solution
specific activity>20,000 units/mg protein
分子量 30.2 kDa (269 amino acids, predicted from the nucleotide sequence)
UniProt登记号P05523
application(s)genomic analysis
运输wet ice
储存温度−20℃
Gene InformationEscherichia coli CFT073 ... mutM(1038243)
Escherichia coli K12 ... mutM(946765)

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 2
个人防护装备Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Sigma-Aldrich

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