产品说明
特异性
Prior to conjugation, the antibody was specific for fibrinogen when tested against human plasma by Coomassie Blue R stained double immunodiffusion and immunoelectrophoresis. Undesired immune reactivity has been removed by solid phase immunoadsorption.
LABELLING RATIO
Fluorescein:antibody molar ratio: 2.8 - 3.3
Absorbance ratio (A493/A276): 0.78 + 0.05
免疫原
Purified fibrinogen derived from pooled human plasma.
应用
Research Category
Signaling
WORKING DILUTION
The final working dilution is dependent on the specific assay conditions. However, in fluorescence microscopy good results can be expected with dilutions in the following ranges:
1. Incident light excitation
Direct immunofluorescence: 1/10 - 1/80
Indirect immunofluorescence: 1/20 - 1/400
2. Transmitted light excitation
Direct immunofluorescence: 1/5 - 1/40
Indirect immunofluorescence: 1/10 - 1/80
Detect Fibrinogend using this Anti-Fibrinogen Antibody, FITC conjugated validated for use in IF, ID & WB.
Research Sub Category
Growth Factors & Receptors
外形
The antibody is supplied in 20mM Tris, 70mM NaCl, pH 8.0, with 0.1% sodium azide as preservative.
储存及稳定性
Store at +2 to +8°C, for up to 6 months, undiluted. Protect from light.
WARNING: This reagent contains 0.1% sodium azide as preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | sheep |
偶联物 | FITC conjugate |
antibody product type | primary antibodies |
克隆 | polyclonal |
species reactivity | human |
manufacturer/tradename | Chemicon® |
technique(s) | immunodiffusion: suitable immunofluorescence: suitable western blot: suitable |
NCBI登记号 | NM_000508.3 NM_021871.2 |
UniProt登记号 | P02671 |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich