Milli-Mark^® Anti-TRA-2-49 Antibody, clone TRA-2-49/6E, FITC Conjugate

产品说明

一般描述

In humans, four isoforms of alkaline phosphatase exist: intestinal, placental, placenta-like (germ cell type) and tissue-nonspecific. The tissue-nonspecific isoform of alkaline phosphatase is present in bone, liver and kidney; it differs from the other forms by posttranslational modification.
Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.

特异性

Antibody recognizes TRA-2-49.

免疫原

2102Ep human Embryonal Carcinoma Cells
Epitope: Unknown

应用

Working Solution	Cells / test	Total Reaction Volume
5 μl	5 x 105 Cells / 95 μl PBS	100 μl
10 μl	1 x 106 Cells / 90 μl PBS	100 μl

Research Sub Category
Pluripotent & Early Differentiation
Detect TRA-2-49 using this Milli-Mark Anti-TRA-2-49 Antibody, clone TRA-2-49/6E, FITC Conjugate validated for use in FC.
Research Category
Stem Cell Research

质量

Evaluated by flow cytometry using 2102 Ep cells.

目标描述

212/80/55 kDa Observed

外形

Protein A purified
Purified mouse monoclonal IgG1 conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.

储存及稳定性

Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.

分析说明

Control
2102 Ep cells.

法律信息

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

产品性质

质量水平	100
生物来源	mouse
偶联物	FITC conjugate
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	TRA-2-49/6E, monoclonal
species reactivity	human
manufacturer/tradename	Milli-Mark®
technique(s)	flow cytometry: suitable
同位素/亚型	IgG1
运输	wet ice

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable