产品说明
特异性
Based on immunoelectrophoresis, the antibody reacts with the heavy chains on human IgG and with light chains common to most human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins, but the antibody may cross-react with immunoglobulins from other species.
免疫原
purified human IgG
应用
Research Sub Category
Fragment Specific Secondary Antibodies
Research Category
Secondary & Control Antibodies
This Goat anti-Human IgG Antibody, F(ab′)2, FITC conjugate is validated for use in IF for the detection of Human IgG.
Immunohistochemistry: 1:500-1:5000
EIA and Western blots: 1:5000-1:100,000
Optimal working dilutions must be determined by end user.
联系
Replaces: AQ316F
外形
Affinity purified antibody. Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15mg/mL BSA, 0.01% thimerosal and 0.05% Sodium Azide
储存及稳定性
Reconstitution: Store freeze-dried product at 4°C until opened for up to 12 months. After opening, restore to 0.8 mL with distilled water to yield 1.4 mg/mL antibody concentration. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. (To judge clarity, draw product into a pasteur pipette). Product is stable for several weeks at 4°C as an undiluted liquid. Discard dilutions after more than one day. For extended storage after reconsitutiton, add an equal volume of glycerol to make a final concentation of 50% glycerol and store at -20°C. Please remember that the concentration of protein (and buffer salts) will decrease to one-half of the original after addition of glycerol.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.46 |
产品性质
质量水平 | 100 |
生物来源 | goat |
偶联物 | FITC conjugate |
抗体形式 | F(ab′)2 fragment of affinity isolated antibody |
antibody product type | secondary antibodies |
克隆 | polyclonal |
species reactivity | human |
manufacturer/tradename | Chemicon® |
technique(s) | immunofluorescence: suitable |
运输 | wet ice |
安全信息
危险声明 | H412 |
预防措施声明 | P273 - P501 |
危险分类 | Aquatic Chronic 3 |
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 3 |
Sigma-Aldrich